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14-3-3 sigma, 1-248aa, Human, E.coli




 

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Product Code: 01-2057S


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20 µg
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Product Details
 
Product Code:01-2057S
Product:14-3-3 sigma, 1-248aa, Human, E.coli
Description:14-3-3 sigma ,also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms:stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma
NCBI Number:NP_006133
Antigen Species:Human
Applications:SDS-PAGE
Form:Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol
Concentration:1 mg/ml (determined by Bradford assay)
Purity:> 95% by SDS - PAGE
Molecular Weight:27.7 kDa (248aa), confirmed by MALDI-TOF.
Amino Acid Sequence:MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS
Gene Category:Apoptosis
Storage:Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References:
  • Benzinger A., et al. (2005) Mol Cell Proteomics. 4(6): 785-95.
  • Wilker E W., et al. (2005) J Biol Chem. 280:18891-18898.
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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