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AAV-CMV-EGFP-P2A-tTA3g-U6 sgRNA




 
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Product Code: ARP-CAS9-17


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Product Code: ARP-CAS9-17
Product: AAV-CMV-EGFP-P2A-tTA3g-U6 sgRNA
Product Category: Cas9 Nuclease Expression Vector_Tet-off Expression System
Description: The vector AAV-TRE-Cas9 is designed for expression of Cas9 nuclease under the TRE promoter control, and the vector AAV-CMV-EGFP--P2A-tTA3g-U6 sgRNA is designed for expression of single-guide RNA and tTA3g which will bind to the TRE promoter to promote the expression of Cas9. A user-defined sgRNA is cloned downstream of a human U6 promoter. To construct the AAV-CMV-EGFP--P2A-tTA3g-U6 sgRNA vector, you must construct pGL3-U6 sgRNA-Puro with your guide sequence. The U6-gRMA sequence is obtained by PCR, and the PCR production is cloned into the pre-linearized vector using the included high-efficiency ligation mix and competent cells. In the presence of the Cas9 endonuclease, sgRNA can recognize the targeted DNA sequence and guide Cas9 nuclease during genome editing for gene knockout, knockin, mutagenesis, and more.
Package Contents: 20 µl AAV-CMV-EGFP-P2A-tTA3g-U6 sgRNA Vector (7.5 ng/µl)
Plasmid Identity: - Vector identity was confirmed by sequencing.
- A260/A280: 1.8–2.0
Storage Buffer: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA (pH 8.0)
Storage: Store at –20°C. Avoid repeated freeze/thaw cycles. Shipped with Ice Gel Packs.
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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