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ANG, Human, ELISA Kit

ANG, Human, ELISA Kit


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Product Code: BEK1007


Product Details Protocol
Product Code:BEK1007
Product:ANG, Human, ELISA Kit
Application:For quantitative detection of ANG in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Background:Angiogenin (ANG) also known as ribonuclease 5, is a potent stimulator of new blood vessel formation. ANG is a member of the pancreatic ribonuclease A superfamily, and RNase activity of ANG is important for its angiogenic activity. It is expressed in the neuroaxis. Like VEGF, ANG is induced by hypoxia to elicit angiogenesis and is expressed in motor neurons. Angiogenin may function as a tRNA-specific ribonuclease that binds to actin on the surface of endothelial cells, once bound, angiogenin is endocytosed and translocated to the nucleus, thereby promoting the endothelial invasiveness necessary for blood vessel formation. Angiogenin induces vascularization of normal and malignant tissues, and abolishes protein synthesis by specifically hydrolyzing cellular tRNAs.
Detection Range:78 pg/ml-5000 pg/ml
Sensitivity:< 12pg/ml
Gene ID:283
Principle of the Assay:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ANG polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ANG polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANG can be calculated.
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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