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Alpha Actinin




 

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Product Code: 03-62002


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250 µg

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Product Details
 
Product Code:03-62002
Product:Alpha Actinin
Description:Alpha Actinin purified from chicken gizzard
Background:Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Synonyms:F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, alpha-actinin-1, non-muscle alpha-actinin-1
Source:Chicken gizzard
Molecular Weight:95,000
Purity:> 95%, SDS-PAGE
Isoelectric Point:pI 5.8
Form:Lyophilized, salt balanced.
Reconstitution:When reconstituted with 250ul H20 dist, a final solution volume of 250 ul will contain 20mM Tris/Acetate, pH 7.6, 0.1mM EDTA, 2mM DTT, 20mM NaCl with a protein concentration of 1mg/ml (according to Bradford protein assay)
Applications:Protein standard in 1D and 2D SDS gelelectrophoresis, Immunoassays, Immunization
Working Dilution:Optimal dilution should be determined by user
Storage:4C (lyophilized); at -20C (solution, aliquot)
References:Duhaiman, A.S. and Bamburg, J.R. (1984) Biochem. 23: 1600
Feramisco, J.R. and Burridge, K. (1980) J. Biol. Chem. 255: 1194
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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