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Anti-Adeno-associated virus 8 (AAV8), intact particles




 

Our Price: $169.00

Product Code: 03-652160


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25 µl

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Product Details
 
Product Code: 03-652160
Product: Anti-Adeno-associated virus 8 (AAV8), intact particles
Description: For characterization of different stages of infection and very useful for analysis of the AAV assembly process. ADK8 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in immunoblotting.
Category: Mouse monoclonal
Clone: ADK8
Ig Subtype: IgG2a, kappa
Background: Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models
Synonyms: AAV8
Immunogen: Adeno-associated virus type 8 (AAV-8) capsid proteins and virus particles
Form: Concentrate of hybridoma culture supernatant
Reactivity: For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK8 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Predicted binding site: residues 586-LQQQNT-591 (cf. Gurda et al.). The antibody cannot be used for immunoblotting using denaturing conditions.
Specificity: Specific for AAV-8, found in monkey. No cross-reaction with other AAV types in immunofluorescence microscopy. With dot blot analysis weak cross-reactivity with type 1, 3, 7 and 10 was observed (Mietzsch et al. 2013).
Applications: Immunofluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Affinity chromatography, Neutralization Assay, ELISA
Working Dilution: Ready-to-use for immunohistochemical applications
Storage: 2-8C
References:
  • Kuck D, Kern A, Kleinschmidt JA (2007). Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J Virol Methods 140, 17-24
  • Sonntag F, Köther K, Schmidt K, Weghofer M, Raupp C, Nieto K, Kuck A, Gerlach B, Böttcher B, Müller OJ, Lux K, Hörer M, Kleinschmidt JA: The assembly-activating protein promotes capsid assembly of different adeno-associated virus serotypes. J Virology 85, 12686-12697 (2011)
  • Gurda BL, Raupp C, Popa-Wagner R, Naumer M, Olson NH, Ng R, McKenna R, Baker TS, Kleinschmidt JA, Agbandje-McKenna M: Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8. J Virology 86, 7739-7751 (2012)
  • Raupp C, Naumer M, Müller OJ, Gurda BL, Agbandje-McKenna M, Kleinschmidt JA: The threefold protrusions of adeno-associated virus type 8 are involved in cell surface targeting as well as postattachment processing. J Virol 17, 9396-9408 (2012); doi:10.1128/JVI.00209-12
  • Mietzsch M, Grasse S, Zurawski C, Weger S, Bennett A, Agbandje-McKenna M, Muzyczka N, Zolotukhin S, Heilbronn R: OneBac:Platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy. Hum Gene Ther 25, 212-222 (2014); doi: 10.1089/hum.2013.184
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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