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Anti-Adeno-associated virus (AAV), intact particles BIOTIN




 

Our Price: $199.00

Product Code: 03-61555


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100 µl

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Product Code: 03-61555
Product: Anti-Adeno-associated virus (AAV), intact particles BIOTIN
Description: For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. The antibody cannot be used for immunoblotting. Specifically detects intact virus particles, both empty and full capsids. With AAV-2 and AAV-3 A20 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins.
Category: Mouse monoclonal
Clone: A20
Ig Subtype: IgG3
Background: Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models
Immunogen: Adeno-associated virus capsid proteins and virus particles
Form: Purified (Protein A), in PBS containing 1% BSA and 0.1% sodium-azide
Reactivity: AAV-2 found in human & monkey. In ELISA also applicable to AAV-3
Specificity: A20 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. Epitope mapping experiments (Wobus et al., see below) identified four immunoreactive (discontinous) regions. The major reaction was attrib- uted to sequence aa 369 to aa378 of AAV-2 capsids. The antibody is also useful for neutralizing experiments (cf. Moskalenko et.al)
Applications: Immunofluorescence microscopy, Immunohistochemistry, ELISA (use PBS with 0.05% Tween 20 as dilution buffer)
Working Dilution: 1:20 for immunofluorescence microscopy and ELISA; 1:5 for immunoprecipitation
Incubation Time: ELISA: 1 hour at 37C
Immunohistochemistry and Immunofluorescence microscopy: Overnight at 2-8C
Storage: 2-8C for immediate use, or at -20C (aliquot)
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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