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Anti-CD31 Monoclonal Antibody




 
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Our Price: $399.00

Product Code: DIA-310


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Product Details
 
Product Code DIA-310
Product: Anti-CD31 Monoclonal Antibody
Description: Anti-mouse CD31 / DIA-310 Rat monoclonal anti-mouse endothelial cell marker CD31 (PECAM-1), Clone SZ31
Category: Monoclonal Antibody
Species: Rat
Clone: SZ31
Immunogen: Murine amino acid fragment
Isotype: IgG2
Species Reactivity: Mouse, Swine - does not cross-react with rat or human
Specificity: CD31
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections.
CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoetic stem cells and embryonic stem cells. CD31 is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 eliminates these restrictions by allowing high quality immunohistochemical analysis of standard formalin-fixed paraffin sections in mice.
Applications: Immunohistochemistry (standard formalin-fixed paraffin and frozen sections), Western blot
Working Dilutions: 1:20 Immunohistochemistry (IHC)
1:5000 Western Blot
Concentration: 0.2 mg/ml
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: The monoclonal antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of aorta, aortic origin, endocardium, small intestine, colon, brain, lymph nodes, bone marrow, mesenteric vessels, kidney, pancreas, lung, muscle, spinal, liver, adenocarcinoma.
References: 1. Hölscher M, Silter M, Krull S, von Ahlen M, Hesse A, Schwartz P, Wielockx B, Breier G, Katschinski DM, Zieseniss A. Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury. J.Biol.Chem. 286: 11185-11194, 2011
2. Kröger C, Vijayaraj P, Reuter U, Windoffer R, Simmons D, Heukamp L, Leube R, Magin TM. Placental vasculogenesis is regulated by keratin-mediated hyperoxia in murine decidual tissues. Am.J.Pathol. 178(4): 1587-1590, 2011
3. Jiang J, Thyagarajan-Sahu A, Krchnák V, Jedinak A, Sandusky GE, et al. NAHA, a Novel Hydroxamic Acid-Derivative, Inhibits Growth and Angiogenesis of Breast Cancer In Vitro and In Vivo. PLoS ONE 7(3): e34283, 2012
4. Copeland BT, Bowman MJ, Ashman LK. Genetic Ablation of the Tetraspanin CD151 Reduces Spontaneous Metastatic Spread of Prostate Cancer in the TRAMP Model. Mol Cancer Res. 11(1):95-105, 2013
5. Agliano A, Martin-Padura I, Marighetti P, Gregato G, Calleri A, Prior C, Redrado M, Calvo A, Bertolini F. Therapeutic effect of lenalidomide in a novel xenograft mouse model of human blastic NK cell lymphoma/blastic plasmacytoid dendritic cell neoplasm. Clin Cancer Res. 1;17(19):6163-73, 2011
6. Ferretti E, Montagna D, Di Carlo E, Cocco C, Ribatti D, Ognio E, Sorrentino C, Lisini D, Bertaina A, Locatelli F, Pistoia V, Airoldi I. Absence of IL-12Rβ2 in CD33(+)CD38(+) pediatric acute myeloid leukemia cells favours progression in NOD/SCID/IL2RϒC-deficient mice. Leukemia 26(2):225-35,2012
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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