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Anti-CD4 Monoclonal Antibody




 
Our Price: $549.00

Product Code: DIA-404


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200 µl

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Product Details
 
Product Code DIA-404
Product: Anti-CD4 Monoclonal Antibody
Description: Anti-mouse CD4 / DIA-404 Rat monoclonal anti-mouse T helper cell marker CD4, Clone GHH4
Category: Monoclonal Antibody
Species: Rat
Clone: GHH4
Immunogen: Recombinant protein (aa 27 - 394 of mouse CD 4)
Isotype: IgG2b kappa
Species Reactivity: Mouse, others not tested.
Specificity: Murine CD4 T helper cells (Th cells)
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone GHH4 has been developed and validated for the detection of murine CD4 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH4 identifies T helper cells in murine FFPE tissue by IHC.
CD4 is a marker for T helper cells. Besides this subpopulation of mature T cells (helper/inducer), CD4 is also expressed on most thymocytes and weakly on macrophages and dendritic cells.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two dif-ferent co-receptors: CD8 and CD4. CD4 is a nonpolymorphous 55 kD glycoprotein belonging to the immunoglobulin superfamily. It acts as a co-receptor with the TCR during antigen-induced T cell activation and thymic differentiation by binding MHC class II and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail.
In humans, CD4 is expressed in the majority of T-cell lymphomas. CD4 plays an important role in the classification of lympho-cytes in inflammatory lesions and malignant lymphomas. CD4 serves as HIV receptor on T cells, macrophages and brain and is downregulated by HIV proteins during AIDS progression.
Applications: Immunohistochemistry (standard formalin-fixed paraffin), others not tested
Working Dilutions: 1:50-1:100 Immunohistochemistry (IHC FFPE)
Concentration: 1 mg/ml
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: Mouse spleen: The monoclonal antibody clone GHH4 specifically stains CD4 in standard FFPE mouse spleen. Incubation with antibody clone GHH4 (10μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with ImmPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Christel Bonnas, Synaptic Systems, Göttingen, Germany).
References: 1. Foti M et al. p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002, 99: 2008-13.
Brady et al. Crystal structure of domains 3 and 4 of rat CD4: relation to the NH2-terminal domains. Science 1993, 260:979-83
2. Janeway CA et al. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992, 10: 645-74.
3. Bierer BE et al. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989, 7: 579-99.
4. Fleischer B et al. Function of the CD4 and CD8 molecules on human T lymphocytes: regulation of T cell triggering. Jour-nal of Immunology 1996, 136: 1625-8
5. Wilde DB et al. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lym-phocyte lines. J Immunol. 1983, 131: 2178-83.
6. Dialynas et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal anti-body GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983, 131: 2445-51.
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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