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BAFF, Human, ELISA Kit




 

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Product Code: BEK1009


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Product Details Protocol
 
Product Code:BEK1009
Product:BAFF, Human, ELISA Kit
Application:For quantitative detection of BAFF in serum, plasma, body fluids, tissue lysates or cell culture supernates.
Background:B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It was expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.As an immunostimulant, BAFF is necessary for maintaining normal immunity.
Detection Range:62.5 pg/ml-4000 pg/ml
Sensitivity:< 2pg/ml
Reactivity:Human
OMIM:603969
SwissProt:Q9Y275
Gene ID:10673
Principle of the Assay:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BAFF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BAFF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BAFF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BAFF can be calculated.
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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