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BMP-7, Human, ELISA Kit




 

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Product Code: BEK1019


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Product Details Protocol
 
Product Code:BEK1019
Product:BMP-7, Human, ELISA Kit
Application:For quantitative detection of BMP-7 in human serum, plasma, urine, bone tissue or cell culture supernatants.
Background:Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a member of the TGF- superfamily. It is expressed in the brain, kidneys and bladder. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes. It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.
Detection Range:31.2 pg/ml-2000 pg/ml
Sensitivity:< 10pg/ml
Reactivity:Human
OMIM:112267
SwissProt:P18075
Gene ID:655
Principle of the Assay:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BMP-7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BMP-7 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-7 can be calculated.
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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