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Bovine 17b-estradiol (17b-E2) ELISA Kit

Bovine 17b-estradiol (17b-E2) ELISA Kit

Our Price: $450.00
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Product Code: KTE10434


Product Details
Product CodeKTE10434
Product:Bovine 17b-estradiol (17b-E2) ELISA Kit
Description:This Bovine 17b-estradiol (17b-E2) ELISA Kit employs a two-site sandwich ELISA to quantitate 17b-E2 in samples. An antibody specific for 17b-E2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any17b-E2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 17b-E2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 17b-E2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background:Estradiol is an estrane (C18) steroid. It is also known as 17b-estradiol (to distinguish it from 17a-estradiol) or as estra-1,3,5(10)-triene-3,17b-diol. It has two hydroxyl groups, one at the C3 position and the other at the 17b position, as well as three double bonds in the A ring. Due to its two hydroxyl groups, estradiol is often abbreviated as E2. Estradiol is the primary female sex hormone.
Synonyms:17b-E2, 17-b-E2
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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