Home > Products > Assay Kits > ELISA >

Bovine Acetylcholine (ACH) ELISA Kit

Bovine Acetylcholine (ACH) ELISA Kit

Our Price: $480.00
Ships in: 5-10 Business Days

Product Code: KTE10124


Product Details
Product CodeKTE10124
Product:Bovine Acetylcholine (ACH) ELISA Kit
Description:This Bovine Acetylcholine (ACH) ELISA Kit employs a two-site sandwich ELISA to quantitate ACH in samples. An antibody specific for ACH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background:Acetylcholine (Ach) is produced by the synthetic enzyme choline acetyltransferase which uses acetyl coenzyme A and choline as substrates for the formation of acetylcholine.Dietary choline and phosphatidylcholine serve as the sources of free choline for acetylcholine synthesis. Upon release, acetylcholine is metabolized into choline and acetate by acetylcholinesterase, and other nonspecific esterases. Acetylcholine release can be excitatory or inhibitory depending on the type of tissue and the nature of the receptor with which it interacts. Cholinergic receptors can be divided into two types, muscarinic and nicotinic, based on the pharmacological action of various agonists and antagonists. Muscarinic receptors originally were distinguished from nicotinic receptors by the selectivity of the agonists muscarine and nicotine respectively.
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:506955
Accession ID:A6QQT9
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

Share your knowledge of this product. Be the first to write a review »