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Bovine Adrenomedullin (ADM) ELISA Kit

Bovine Adrenomedullin (ADM) ELISA Kit


 
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Product Code: KTE10127


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Product Details
 
Product CodeKTE10127
Product:Bovine Adrenomedullin (ADM) ELISA Kit
Description:This Bovine Adrenomedullin (ADM) ELISA Kit employs a two-site sandwich ELISA to quantitate ADM in samples. An antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Species:Bovine
Background:Adrenomedullin (ADM) is a peptide associated with pheochromocytoma. Adrenomedullin (AM) is a ubiquitously expressed peptide initially isolated from phaechromyctoma. Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. The human AM gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns.
Synonyms:ADM, AM, preproadrenomedullin
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:280713
Accession ID:O62827
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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