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Bovine Apoptosis regulator BAX (BAX) ELISA Kit

Bovine Apoptosis regulator BAX (BAX) ELISA Kit

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Product Code: KTE10421


Product Details
Product CodeKTE10421
Product:Bovine Apoptosis regulator BAX (BAX) ELISA Kit
Description:This Bovine Apoptosis regulator BAX (BAX) ELISA Kit employs a two-site sandwich ELISA to quantitate BAX in samples. An antibody specific for BAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BAX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background:Bax (Bcl-2 associated x protein) is an apoptosis regulating protein of the Bcl-2 family. The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stimulus. Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology. Bax is a 21 kDa protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo.Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade.
Synonyms:BAX, BCL2L4, apoptosis regulator BAX
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:280730
Accession ID:O02703
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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