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Bovine Beta-Hydroxybutyric Acid (B-OHB) ELISA Kit

Bovine Beta-Hydroxybutyric Acid (B-OHB) ELISA Kit

Our Price: $505.00
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Product Code: KTE10422


Product Details
Product CodeKTE10422
Product:Bovine Beta-Hydroxybutyric Acid (B-OHB) ELISA Kit
Description:This Bovine Beta-Hydroxybutyric Acid (B-OHB) ELISA Kit employs a two-site sandwich ELISA to quantitate B-OHB in samples. An antibody specific for B-OHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB-OHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B-OHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B-OHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background:Beta-Hydroxybutyric acid is a ketone body. It is a chiral compound having two enantiomers,ξD-3-hydroxybutyric acid andξL-3-hydroxybutyric acid. Like the other ketone bodies, levels ofξbeta-hydroxybutyrate are raised in ketosis. In humans,ξbeta-hydroxybutyrate is synthesized in the liver from acetyl-CoA, and can be used as an energy source by the brain when blood glucose is low. Diabetic patients can have their ketone levels tested via urine or blood to indicate diabetic ketoacidosis. In alcoholic ketoacidosis, this ketone body is produced in greatest concentration. Both types of ketoacidosis result in an increase B-Hydroxybutyrate to oxaloacetate ratio, resulting in TCA cycle stalling and shifting of glucose towards ketone body production.Industrially, it can also be used for the synthesis of biodegradable plastics, such as poly. This polymer can be produced biologically by the bacteria, Alcaligenes eutrophus.
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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