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Bovine Cardiac Troponin T (cTn-T) ELISA Kit

Bovine Cardiac Troponin T (cTn-T) ELISA Kit

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Product Code: KTE10023


Product Details
Product CodeKTE10023
Product:Bovine Cardiac Troponin T (cTn-T) ELISA Kit
Description:This Bovine Cardiac Troponin T (cTn-T) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNC1 in samples. An antibody specific for TNNC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background:Troponin is a component of thin filaments (along with actin and tropomyosin), and is the protein complex to which calcium binds to trigger the production of muscular force. Troponin itself has three subunits, TnC, TnI, and TnT, each playing a role in force regulation. TnT is a tropomyosin-binding subunit which regulates the interaction of troponin complex with thin filaments. cTnT is probably less cardiac specific. Expression of cTnT in skeletal tissue of patients with chronic skeletal muscle injuries has been described.
Synonyms:TNNC1, CMD1Z, TNC, TNNC, cardiac troponin C, slow twitch skeletal/cardiac muscle troponin C, troponin C, slow, troponin C1, slow
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:509486
Accession ID:P63315
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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