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Chromatrap ChIP qPCR Protein G Kit

Chromatrap ChIP qPCR Protein G Kit

Our Price: $489.00
Ships in: Ships in 3-5 Business Days

Product Code: 500117

24 Sample Kit

Product Details
Product Code 500117
Product: Chromatrap ChIP qPCR Protein G Kit
Description: QPCR Standard Chromatrap Pro-G spin column kit for 24 samples (no control antibodies or primers)
Background: Chromatrap® ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The kits have been optimised for 1000ng sample sizes, which allows for better results from smaller sample sizes and greater flexibility as more IP assays can be performed from one sample.
  • Designed for qPCR as a downstream process only
  • Suitable for low chromatin loadings (1µg-10µg)
  • Contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitations
  • Up to 10 chromatin preparations by sanitation
  • An enzymatic shearing format is also available
The kit is not suitable for sequencing.
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Hypotonic Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K and 1.5ml Collection tubes.
Protocol: Download Protocol
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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