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Chromatrap Enzymatic ChIP qPCR Protein G Kit




 
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Product Code: 500168


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24 Sample Kit

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Product Details
 
Product Code 500168
Product: Chromatrap Enzymatic ChIP qPCR Protein G Kit
Description: QPCR Enzymatic Standard Chromatrap Pro-G spin column kit for 24 samples (no control antibodies or primers)
Background: Chromatrap® Enzymatic ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The Enzymatic ChIP kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40µls) with no DNA clean up steps prior to qPCR. This kit is designed for use as qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1µg-10µg)
Features:
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit contains sufficient Protein-A or Protein-G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and reagents for enzymatic shearing of chromatin (up to 10 preparations)
  • A high throughput format is also available allowing for multiple sample type, antibody and gene targets to be tested in parallel
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Digestion Buffer, Enzymatic Stop Solution, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes.
Protocol: Download Protocol
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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