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Chromatrap Enzymatic HT ChIP-qPCR Protein A Kit




 
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Product Code: 500162


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96 Well Plate

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Product Details
 
Product Code 500162
Product: Chromatrap Enzymatic HT ChIP-qPCR Protein A Kit
Description: QPCR Enzymatic Standard Chromatrap Pro-A high throughput ChIP 96 well Microplate
Background: Chromatin can be sheared enzymatically or by mechanical methods such as sonication. The Enzymatic ChIP 96 microplate kit is a perfect alternative for those customers who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The Chromatrap® 96 high throughput ChIP microplate for qPCR is based on the novel spin-column ChIP technique.

The kit contains 96 Protein-A or Protein-G based filters in one SLAS/ANSI standard microplate footprint device, with buffers and reagents to perform up to 96 chromatin immunoprecipitation assays and up to 10 chromatin preparations by enzymatic digestion.

For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.

For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.
Features:
  • Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.
  • This kit is designed for qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1-10µg).
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample.
  • The kit is also available for sequencing as a downstream process.
Kit Contents: Chromatrap® 96 HT Protein A, Chromatrap® 96 HT balance plate, 96- well collection plate, 96- well balance collection plate, 96- well elution plate, 96- well balance elution plate, Elution and balance plate strip caps, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Digestion Buffer, Enzymatic stop solution, Lysis Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K.
Protocol: Download Protocol
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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