Home > Suppliers > Chromatrap >

Chromatrap FFPE ChIP-seq Protein A Kit




 
Our Price: $839.00
Ships in: Ships in 3-5 Business Days

Product Code: 500235


Size*:
24 Columns

Qty:
Product Details
 
Product Code 500235
Product: Chromatrap FFPE ChIP-seq Protein A Kit
Description: qPCR from FFPE Chromatrap Pro-A spin column kit, 24 columns (includes control antibodies and enzymes)
Background: A solid phase chromatin immunoprecipitation assay from chromatin extracted from Formalin Fixed Paraffin Embedded (FFPE) tissue. The Chromatrap® FFPE ChIP kit has overcome the issues often associated with FFPE ChIP, by offering a streamlined protocol which provides the user with good quality isolated chromatin.

Ideal for tissue samples, biopsies and difficult to lyse samples. The sensitivity of the unique solid state platform ensures that small samples can be used with efficient enrichment of both high and low abundant targets. Chromatrap® FFPE ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from a range of FFPE samples. Using the unique, patented Chromatrap® technology maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
Features:
  • This kit is compatible with both qPCR and sequencing as a downstream process and has a wide dynamic range, ensuring that adequate DNA is enriched for library preparation for sequencing. The wide dynamic range allows for better results from small sample sizes and provides greater flexibility
  • Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
  • The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
Kit Contents: Chromatrap® Protein G spin columns (24), Paraffin Removal Solution, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, Digestion Buffer, Extraction Buffer, Enzymatic Stop Solution, FFPE Elution solution, 5M NaCl, 1M NaHCO3, FFPE Lysis buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer.
Protocol: Download Protocol
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

Share your knowledge of this product. Be the first to write a review »