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Chromatrap HT Enzymatic ChIP-seq Protein A Kit




 
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Product Code: 500216


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96 Well Plate

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Product Details
 
Product Code 500216
Product: Chromatrap HT Enzymatic ChIP-seq Protein A Kit
Description: ChIP-seq Enzymatic 96 well plate kit, Pro A, with Premium control antibodies
Background: Chromatrap® Enzymatic ChIP-Seq 96 well Microplane kit combines the solid state technology in 96 well format with high throughput sequencing to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations.

The unrivalled flexibility of experimental design offered by Chromatrap® ChIP-Seq 96 allows large reliable data sets to be collected efficiently enabling widespread effects to be studies across multiple sample and cell types all carried out simultaneously.

The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion. The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation. Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation. With selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process and has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
  • The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion
  • The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation
Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation.
Kit Contents: Chromatrap® 96 HT Protein A plate, Chromatrap® 96 HT balance plate, 96-well collection plate, 96-well balance collection plate, Elution plate, 96-well balance elution plate, Elution and balance plate strip caps, Column conditioning buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Digestion Buffer, Enzymatic Stop Solution, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K. DNA purification plate, DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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