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Chromatrap Native ChIP-seq Protein A Kit




 
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Product Code: 500237


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24 Columns

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Product Details
 
Product Code 500237
Product: Chromatrap Native ChIP-seq Protein A Kit
Description: qPCR & Sequencing Chromatrap Native Pro-A ChIP kit for enzymatic shearing only
Background: Chromatrap® Native ChIP kits provide an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction.

In native chromatin the proteins and histones wrapped around DNA are naturally linked and the traditional methods of shearing can easily disrupt the DNA- protein complexes. Therefore the DNA is biologically sheared using enzyme digestion of the chromatin. This kit can be can be used for the study of histone modifications and certain abundant transcription factors that are likely to be bound to DNA.

Chromatrap® Native ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from native chromatin. Using the unique patented technology maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process and has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility, thus more IP assays can be performed from just one sample.
  • The kit is ideal for the enrichment of histone modifications and some low abundant transcription factors.
  • Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
  • The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, N-ChIP Digestion Buffer, ChIP Dialysis Buffer, Resuspension Buffer, Separating Solution 1, Separating Solution 2, Enzymatic Stop Solution, N-ChIP Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer.
Protocol: Download Protocol
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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