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Chromatrap® provides a revolutionary solid state platform for chromatin immunoprecipitation (ChIP) using spin columns or filter plates. Chromatrap® continues to help researchers get more from their epigenetics research.

Compared to standard methods, Chromatrap® is:

Faster
More robust
Easier to use
More sensitive, especially for low-abundant targets

What is ChIP?
ChIP is a technique used to study the association of specific proteins, or their modified isoforms, with defined genomic regions. It is a fast growing research technique and is commonly used for mapping the DNA-protein interactions in cells which are crucial for correct gene regulation. For example, they may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region of DNA of living cells or tissues.

In a ChIP assay, fragments of the DNA-protein complex (chromatin) are cross-linked in such a way so as to retain the specific DNA-protein interactions. The chromatin is then extracted and sheared either by sonication or enzymatic digestion into small fragments. The DNA/protein fragments are selectively immunoprecipitated using antibodies directed against the protein of interest and the resulting fractions treated to separate the DNA and protein components.Why is the Chromatrap® technology better?

Chromatrap® kits use spin columns or filter-bottom microplates which contain discs of a novel, inert, porous polymer to which Protein A or G has been covalently attached. This patented format is unique and has been granted in the UK (Patent No. GB2482209), the US (Patent No. 9523681) and China (Patent No. ZL 2011 8 0067254.X).

During an assay, the chromatin/antibody complex is selectively retained by the disc. Washing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory.

Advantages of Chromatrap®:
ChIP in under 5 hours from chromatin preparation to qPCR
Less manual handling, eliminating sample loss through multiple pipetting steps
Single column and 96-well high throughput formats available
Optimised for 1000 ng sample sizes, as smaller samples give better qPCR results
Allows more IP assays from a single sample

How does it perform in the laboratory?
Chromatrap® outperforms other chromatin immunoprecipitation assays because it has:
Robust signal to noise- typically 2-3 times better than standard methods
Low non-specific binding on the inert Chromatrap® discs
No DNA clean-up required for qPCR
High surface area and molecular mixing ideal for low abundant targets and challenging cell types
Wide dynamic range makes it suitable for ChIP-seq
96 well format for high throughput analysis
Allows more ChIP assays to be performed from a single sample

The novel solid state Chromatrap® protocols do differ from those used by other kit suppliers, including Active Motif, Diagenode, Cell Signalling Technology (CST), Abcam, Zymo Research and Epigentek, as these are all bead-based kits. It is therefore imperative that you carefully study the protocols used by Chromatrap first. We do not advise mixing buffers or washes from other manufacturers’ kits with Chromatrap® columns, as our superior solid-state chemistry may not be compatible with their kit components.

Need help deciding which Chromatrap product is most suitable for your needs?
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500071
Chromatrap ChIP qPCR Protein A Kit
Product Code 500071
Product: Chromatrap ChIP qPCR Protein A Kit
Description: QPCR Standard Chromatrap Pro-A spin column kit for 24 samples (no control antibodies or primers)
Background: Chromatrap® ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The kits have been optimised for 1000ng sample sizes, which allows for better results from smaller sample sizes and greater flexibility as more IP assays can be performed from one sample.
Features:
  • Designed for qPCR as a downstream process only
  • Suitable for low chromatin loadings (1µg-10µg)
  • Contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitations
  • Up to 10 chromatin preparations by sanitation
  • An enzymatic shearing format is also available
The kit is not suitable for sequencing.
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Hypotonic Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K and 1.5ml Collection tubes.
Protocol: Download Protocol

Our Price: $489.00
500117
Chromatrap ChIP qPCR Protein G Kit
Product Code 500117
Product: Chromatrap ChIP qPCR Protein G Kit
Description: QPCR Standard Chromatrap Pro-G spin column kit for 24 samples (no control antibodies or primers)
Background: Chromatrap® ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The kits have been optimised for 1000ng sample sizes, which allows for better results from smaller sample sizes and greater flexibility as more IP assays can be performed from one sample.
Features:
  • Designed for qPCR as a downstream process only
  • Suitable for low chromatin loadings (1µg-10µg)
  • Contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitations
  • Up to 10 chromatin preparations by sanitation
  • An enzymatic shearing format is also available
The kit is not suitable for sequencing.
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Hypotonic Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K and 1.5ml Collection tubes.
Protocol: Download Protocol

Our Price: $489.00
500189
Chromatrap ChIP-seq Pro A Kit
Product Code 500189
Product: Chromatrap ChIP-seq Pro A Kit
Description: ChIP-seq 24 spin columns, Pro A, with Premium control antibodies
Background: Chromatrap®’s new ChIP-Seq kit utilises the solid state technology to deliver a streamlined ChIP-Seq protocol from small cell numbers and low chromatin concentrations.

Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-Seq now combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of transcription factor binding sites and specific DNA associated protein modifications. The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors.

The ChIP-Seq kit is our more sensitive kit especially for low abundant transcription factors, and can be used for both qPCR and sequencing. This kit is compatible with both high and low chromatin loadings with an increased slurry volume of 1ml offering greater flexibility with more difficult samples. This kit requires a clean-up step but due to the increased binding of the column sufficient DNA is obtained from one column without the need to pool samples for library preparation.
Features:
  • Compatible for both qPCR and sequencing as a downstream process
  • Has a wide dynamic range suitable for low and high chromatin loadings (1-50µg), which allows for better results from small sample sizes and providers greater flexibility
  • Allows user to perform up to 24 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations.
  • Kit provides all the major components required for performing ChIP assays and DNA purification columns to obtain high quality DNA for NGS library preparation
  • Positive and negative antibody and primer sets are provided in the kit
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA Binding Buffer, DNA Wash Buffer and DNA Elution Buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $649.00
500190
Chromatrap ChIP-seq Pro G Kit
Product Code 500190
Product: Chromatrap ChIP-seq Pro G Kit
Description: ChIP-seq 24 spin columns, Pro G, with Premium control antibodies
Background: Chromatrap®’s new ChIP-Seq kit utilises the solid state technology to deliver a streamlined ChIP-Seq protocol from small cell numbers and low chromatin concentrations.

Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-Seq now combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of transcription factor binding sites and specific DNA associated protein modifications. The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors.

The ChIP-Seq kit is our more sensitive kit especially for low abundant transcription factors, and can be used for both qPCR and sequencing. This kit is compatible with both high and low chromatin loadings with an increased slurry volume of 1ml offering greater flexibility with more difficult samples. This kit requires a clean-up step but due to the increased binding of the column sufficient DNA is obtained from one column without the need to pool samples for library preparation.
Features:
  • Compatible for both qPCR and sequencing as a downstream process
  • Has a wide dynamic range suitable for low and high chromatin loadings (1-50µg), which allows for better results from small sample sizes and providers greater flexibility
  • Allows user to perform up to 24 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations.
  • Kit provides all the major components required for performing ChIP assays and DNA purification columns to obtain high quality DNA for NGS library preparation
  • Positive and negative antibody and primer sets are provided in the kit
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes. DNA purification columns (24), DNA Binding Buffer, DNA Wash Buffer and DNA Elution Buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $649.00
500240
Chromatrap DNA Clean and Concentrate HT Kit
Product Code 500240
Product: Chromatrap DNA Clean and Concentrate HT Kit
Description: The Chromatrap® DNA microelution kit recovers ultra-pure DNA from ChIP samples, it also recovers any DNA that has been mechanically or enzymatically sheared. This kit can be used to concentrate samples due to low elution volumes, as little as 5µl.

Micro-elution DNA PCR Clean Up- 30% Silica, 96 wells, pack of 2
Background: Chromatrap® provide 96 well high throughput plates for ultra-pure DNA purification. Using proprietary filtration media that offer much higher loadings of active material, assay times are under 5 minutes.

Buffers are optimised to remove any unwanted impurities while providing efficient DNA recovery from samples. The Chromatrap® DNA kit is designed for the purification and concentration of samples from PCR mixtures, ChIP and restriction enzyme digestions.
Features:
  • DNA samples ranging from 50 bp up to 23 kb can be purified with up to 98% recovery.
  • Up to 50µg of DNA can be recovered efficiently and quickly using a Chromatrap® DNA purification kit.
Kit Contents: 2 x 96 microelution DNA purification plates, DNA binding buffer, DNA wash buffer, DNA elution buffer and 2 x 96 DNA elution plates.
Protocol: Download Protocol

Our Price: $399.00
500220
Chromatrap DNA Purification HT Kit
Product Code 500220
Product: Chromatrap DNA Purification HT Kit
Description: DNA PCR 96 well plate clean up kit (30%) - Pack of 2
Background: Chromatrap® provide 96 well high throughput plates for ultra-pure DNA purification. Using proprietary filtration media that offer much higher loadings of active material, assay times are under 5 minutes.

Buffers are optimised to remove any unwanted impurities while providing efficient DNA recovery from samples. The Chromatrap® DNA kit is designed for the purification and concentration of samples from PCR mixtures, ChIP and restriction enzyme digestions.
Features:
  • DNA samples ranging from 50 bp up to 23 kb can be purified with up to 98% recovery.
  • Up to 50µg of DNA can be recovered efficiently and quickly using a Chromatrap® DNA purification kit.
Kit Contents: 2 x 96 DNA purification plates, DNA binding buffer, DNA wash buffer, DNA elution buffer and 2 x 96 DNA elution plates.
Protocol: Download Protocol

Our Price: $399.00
500166
Chromatrap Enzymatic ChIP qPCR Protein A Kit
Product Code 500166
Product: Chromatrap Enzymatic ChIP qPCR Protein A Kit
Description: QPCR Enzymatic Standard Chromatrap Pro-A spin column kit for 24 samples (no control antibodies or primers)
Background: Chromatrap® Enzymatic ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The Enzymatic ChIP kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40µls) with no DNA clean up steps prior to qPCR. This kit is designed for use as qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1µg-10µg)
Features:
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit contains sufficient Protein-A or Protein-G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and reagents for enzymatic shearing of chromatin (up to 10 preparations)
  • A high throughput format is also available allowing for multiple sample type, antibody and gene targets to be tested in parallel
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Digestion Buffer, Enzymatic Stop Solution, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes.
Protocol: Download Protocol

Our Price: $589.00
500168
Chromatrap Enzymatic ChIP qPCR Protein G Kit
Product Code 500168
Product: Chromatrap Enzymatic ChIP qPCR Protein G Kit
Description: QPCR Enzymatic Standard Chromatrap Pro-G spin column kit for 24 samples (no control antibodies or primers)
Background: Chromatrap® Enzymatic ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The Enzymatic ChIP kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40µls) with no DNA clean up steps prior to qPCR. This kit is designed for use as qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1µg-10µg)
Features:
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit contains sufficient Protein-A or Protein-G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and reagents for enzymatic shearing of chromatin (up to 10 preparations)
  • A high throughput format is also available allowing for multiple sample type, antibody and gene targets to be tested in parallel
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Digestion Buffer, Enzymatic Stop Solution, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes.
Protocol: Download Protocol

Our Price: $589.00
500191
Chromatrap Enzymatic ChIP-seq Protein A Kit
Product Code 500191
Product: Chromatrap Enzymatic ChIP-seq Protein A
Description: ChIP-seq Enzymatic 24 spin columns, Pro A, with Premium control antibodies
Background: Chromatrap®’s new ChIP-Seq kit utilises the solid state technology to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations.

Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-seq now combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of transcription factor binding sites and specific DNA associated protein modifications.

The Enzymatic ChIP-seq kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The ChIP-Seq kit is our more sensitive kit and can be used for both qPCR and sequencing. This kit is compatible with both high and low chromatin loadings with an increased slurry volume of 1ml offering greater flexibility with more difficult samples. This kit requires a clean-up step but due to the increased binding of the column sufficient DNA is obtained from one column without the need to pool samples for library preparation.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process
  • Wide dynamic range suitable for low and high chromatin loadings (1-50µg) and the wide dynamic range allows for better results from small sample sizes and provides greater flexibility
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
  • Allows the user to perform up to 24 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations
  • Kit provides all of the major components required for performing ChIP assays and DNA purification columns to obtain high quality DNA for NGS library preparation
  • Positive and negative antibody and primer sets are provided in the kit
  • Selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Digestion Buffer, Enzymatic Stop Solution, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes, DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $719.00
500192
Chromatrap Enzymatic ChIP-seq Protein G Kit
Product Code 500192
Product: Chromatrap Enzymatic ChIP-seq Protein G
Description: ChIP-seq Enzymatic 24 spin columns, Pro G, with Premium control antibodies
Background: Chromatrap®’s new ChIP-Seq kit utilises the solid state technology to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations.

Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-seq now combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of transcription factor binding sites and specific DNA associated protein modifications.

The Enzymatic ChIP-seq kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The ChIP-Seq kit is our more sensitive kit and can be used for both qPCR and sequencing. This kit is compatible with both high and low chromatin loadings with an increased slurry volume of 1ml offering greater flexibility with more difficult samples. This kit requires a clean-up step but due to the increased binding of the column sufficient DNA is obtained from one column without the need to pool samples for library preparation.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process
  • Wide dynamic range suitable for low and high chromatin loadings (1-50µg) and the wide dynamic range allows for better results from small sample sizes and provides greater flexibility
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
  • Allows the user to perform up to 24 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations
  • Kit provides all of the major components required for performing ChIP assays and DNA purification columns to obtain high quality DNA for NGS library preparation
  • Positive and negative antibody and primer sets are provided in the kit
  • Selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Digestion Buffer, Enzymatic Stop Solution, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes, DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $719.00
500162
Chromatrap Enzymatic HT ChIP-qPCR Protein A Kit
Product Code 500162
Product: Chromatrap Enzymatic HT ChIP-qPCR Protein A Kit
Description: QPCR Enzymatic Standard Chromatrap Pro-A high throughput ChIP 96 well Microplate
Background: Chromatin can be sheared enzymatically or by mechanical methods such as sonication. The Enzymatic ChIP 96 microplate kit is a perfect alternative for those customers who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The Chromatrap® 96 high throughput ChIP microplate for qPCR is based on the novel spin-column ChIP technique.

The kit contains 96 Protein-A or Protein-G based filters in one SLAS/ANSI standard microplate footprint device, with buffers and reagents to perform up to 96 chromatin immunoprecipitation assays and up to 10 chromatin preparations by enzymatic digestion.

For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.

For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.
Features:
  • Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.
  • This kit is designed for qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1-10µg).
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample.
  • The kit is also available for sequencing as a downstream process.
Kit Contents: Chromatrap® 96 HT Protein A, Chromatrap® 96 HT balance plate, 96- well collection plate, 96- well balance collection plate, 96- well elution plate, 96- well balance elution plate, Elution and balance plate strip caps, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Digestion Buffer, Enzymatic stop solution, Lysis Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K.
Protocol: Download Protocol

Our Price: $1,229.00
500164
Chromatrap Enzymatic HT ChIP-qPCR Protein G Kit
Product Code 500164
Product: Chromatrap Enzymatic HT ChIP-qPCR Protein G Kit
Description: QPCR Enzymatic Standard Chromatrap Pro-G high throughput ChIP 96 well Microplate
Background: Chromatin can be sheared enzymatically or by mechanical methods such as sonication. The Enzymatic ChIP 96 microplate kit is a perfect alternative for those customers who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The Chromatrap® 96 high throughput ChIP microplate for qPCR is based on the novel spin-column ChIP technique.

The kit contains 96 Protein-A or Protein-G based filters in one SLAS/ANSI standard microplate footprint device, with buffers and reagents to perform up to 96 chromatin immunoprecipitation assays and up to 10 chromatin preparations by enzymatic digestion.

For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.

For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.
Features:
  • Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.
  • This kit is designed for qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1-10µg).
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample.
  • The kit is also available for sequencing as a downstream process.
This kit is not suitable for sequencing.
Kit Contents: Chromatrap® 96 HT Protein G, Chromatrap® 96 HT balance plate, 96- well collection plate, 96- well balance collection plate, 96- well elution plate, 96- well balance elution plate, Elution and balance plate strip caps, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Digestion Buffer, Enzymatic stop solution, Lysis Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K.
Protocol: Download Protocol

Our Price: $1,229.00
500165
Chromatrap Enzymatic Shearing Kit
Product Code 500165
Product: Chromatrap Enzymatic Shearing Kit
Description: Chromatrap Enzymatic Shearing Buffers Kit
Background: The Chromatrap® Enzymatic Shearing Kit provides an excellent method for the preparation of high quality, ideally fragmented chromatin for ChIP analysis.

The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The Enzymatic shearing kit provides buffers to optimise the shearing conditions of your chromatin by enzymatic digestion.

Chromatin prepared using this kit demonstrates excellent enrichment, independent of starting cell number. With its quick and simple protocol the Chromatrap® Enzymatic Shearing Kit is the perfect cost-effective alternative to sonication.
Features:
  • Supplies all reagents and buffers for up to 10 chromatin preparations, which determines optimal shearing conditions
  • Generates enough chromatin to perform up to 24 ChIPs using a standard Chromatrap® ChIP spin column kit or up to 96 IPs using the Chromatrap® 96 HT microplate kit
Kit Contents: 1.3M Glycine, Hypotonic Buffer, Digestion Buffer, Enzymatic Stop Solution, Lysis Buffer, 5M NaCl, 1M NaHCO3, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K and Proteinase K Stop Solution.
Protocol: Download Protocol

Our Price: $289.00
500235
Chromatrap FFPE ChIP-seq Protein A Kit
Product Code 500235
Product: Chromatrap FFPE ChIP-seq Protein A Kit
Description: qPCR from FFPE Chromatrap Pro-A spin column kit, 24 columns (includes control antibodies and enzymes)
Background: A solid phase chromatin immunoprecipitation assay from chromatin extracted from Formalin Fixed Paraffin Embedded (FFPE) tissue. The Chromatrap® FFPE ChIP kit has overcome the issues often associated with FFPE ChIP, by offering a streamlined protocol which provides the user with good quality isolated chromatin.

Ideal for tissue samples, biopsies and difficult to lyse samples. The sensitivity of the unique solid state platform ensures that small samples can be used with efficient enrichment of both high and low abundant targets. Chromatrap® FFPE ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from a range of FFPE samples. Using the unique, patented Chromatrap® technology maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
Features:
  • This kit is compatible with both qPCR and sequencing as a downstream process and has a wide dynamic range, ensuring that adequate DNA is enriched for library preparation for sequencing. The wide dynamic range allows for better results from small sample sizes and provides greater flexibility
  • Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
  • The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
Kit Contents: Chromatrap® Protein G spin columns (24), Paraffin Removal Solution, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, Digestion Buffer, Extraction Buffer, Enzymatic Stop Solution, FFPE Elution solution, 5M NaCl, 1M NaHCO3, FFPE Lysis buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer.
Protocol: Download Protocol

Our Price: $839.00
500236
Chromatrap FFPE ChIP-seq Protein G Kit
Product Code 500236
Product: Chromatrap FFPE ChIP-seq Protein G Kit
Description: qPCR from FFPE Chromatrap Pro-G spin column kit, 24 columns (includes control antibodies and enzymes)
Background: A solid phase chromatin immunoprecipitation assay from chromatin extracted from Formalin Fixed Paraffin Embedded (FFPE) tissue. The Chromatrap® FFPE ChIP kit has overcome the issues often associated with FFPE ChIP, by offering a streamlined protocol which provides the user with good quality isolated chromatin.

Ideal for tissue samples, biopsies and difficult to lyse samples. The sensitivity of the unique solid state platform ensures that small samples can be used with efficient enrichment of both high and low abundant targets. Chromatrap® FFPE ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from a range of FFPE samples. Using the unique, patented Chromatrap® technology maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
Features:
  • This kit is compatible with both qPCR and sequencing as a downstream process and has a wide dynamic range, ensuring that adequate DNA is enriched for library preparation for sequencing. The wide dynamic range allows for better results from small sample sizes and provides greater flexibility
  • Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
  • The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
Kit Contents: Chromatrap® Protein G spin columns (24), Paraffin Removal Solution, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, Digestion Buffer, Extraction Buffer, Enzymatic Stop Solution, FFPE Elution solution, 5M NaCl, 1M NaHCO3, FFPE Lysis buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer.
Protocol: Download Protocol

Our Price: $839.00
500161
Chromatrap HT ChIP-qPCR Protein A Kit
Product Code 500161
Product: Chromatrap HT ChIP-qPCR Protein A Kit
Description: QPCR Standard Chromatrap Pro-A high throughput ChIP 96 well Microplate
Background: The Chromatrap® 96 high throughput ChIP microplate for qPCR is based on the novel spin-column ChIP technique. The kit contains 96 Protein A or Protein G based filters in one SLAS/ANSI standard microplate footprint device, with buffers and reagents to perform up to 96 chromatin immunoprecipitation assays and up to 10 chromatin preparations by sonication.

Chromatrap®96 ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR. For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.
Features:
  • Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.
  • Designed for use as qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1-10µg).
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample.
  • The kit is also available in enzymatic and sequencing format.
Kit Contents: Chromatrap® 96 HT Protein A microplate, Chromatrap® 96 HT balance plate, 96- well collection plate, 96- well balance collection plate, 96- well elution plate, 96- well balance elution plate, Elution and balance plate strip caps, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, and Proteinase K.
Protocol: Download Protocol

Our Price: $1,149.00
500214
Chromatrap HT ChIP-seq Pro A Kit
Product Code 500214
Product: Chromatrap HT ChIP-seq Pro A Kit
Description: ChIP-seq 96 well plate kit, Pro A, with Premium control antibodies
Background: Chromatrap® ChIP-Seq 96 well Microplate sonication kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations.

The kit provides all of the major components required for performing ChIP assays and DNA purification plate to obtain high quality DNA for NGS library preparation. Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation. With selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA.
Features:
  • This kit is compatible with both qPCR and sequencing as a downstream process
  • Has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
Kit Contents: Chromatrap® 96 HT Protein A plate, Chromatrap® 96 HT balance plate, 96-well collection plate, 96-well balance collection plate, Elution plate,, 96-well balance elution plate, Elution and balance plate strip caps, Column conditioning buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K. DNA purification plate, DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $1,399.00
500215
Chromatrap HT ChIP-seq Pro G Kit
Product Code 500215
Product: Chromatrap HT ChIP-seq Pro G Kit
Description: ChIP-seq 96 well plate kit, Pro G, with Premium control antibodies
Background: Chromatrap® ChIP-Seq 96 well Microplate sonication kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations.

The kit provides all of the major components required for performing ChIP assays and DNA purification plate to obtain high quality DNA for NGS library preparation. Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation. With selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA.
Features:
  • This kit is compatible with both qPCR and sequencing as a downstream process
  • Has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
Kit Contents: Chromatrap® 96 HT Protein G plate, Chromatrap® 96 HT balance plate, 96-well collection plate, 96-well balance collection plate, Elution plate, 96-well balance elution plate, Elution and balance plate strip caps, Column conditioning buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K. DNA purification plate, DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $1,399.00
500216
Chromatrap HT Enzymatic ChIP-seq Protein A Kit
Product Code 500216
Product: Chromatrap HT Enzymatic ChIP-seq Protein A Kit
Description: ChIP-seq Enzymatic 96 well plate kit, Pro A, with Premium control antibodies
Background: Chromatrap® Enzymatic ChIP-Seq 96 well Microplane kit combines the solid state technology in 96 well format with high throughput sequencing to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations.

The unrivalled flexibility of experimental design offered by Chromatrap® ChIP-Seq 96 allows large reliable data sets to be collected efficiently enabling widespread effects to be studies across multiple sample and cell types all carried out simultaneously.

The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion. The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation. Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation. With selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process and has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
  • The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion
  • The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation
Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation.
Kit Contents: Chromatrap® 96 HT Protein A plate, Chromatrap® 96 HT balance plate, 96-well collection plate, 96-well balance collection plate, Elution plate, 96-well balance elution plate, Elution and balance plate strip caps, Column conditioning buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Digestion Buffer, Enzymatic Stop Solution, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K. DNA purification plate, DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $1,419.00
500217
Chromatrap HT Enzymatic ChIP-seq Protein G Kit
Product Code 500217
Product: Chromatrap HT Enzymatic ChIP-seq Protein G Kit
Description: ChIP-seq Enzymatic 96 well plate kit, Pro G, with Premium control antibodies
Background: Chromatrap® Enzymatic ChIP-Seq 96 well Microplane kit combines the solid state technology in 96 well format with high throughput sequencing to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations.

The unrivalled flexibility of experimental design offered by Chromatrap® ChIP-Seq 96 allows large reliable data sets to be collected efficiently enabling widespread effects to be studies across multiple sample and cell types all carried out simultaneously.

The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion. The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation. Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation. With selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process and has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
  • The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion
  • The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation
Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation.
Kit Contents: Chromatrap® 96 HT Protein G plate, Chromatrap® 96 HT balance plate, 96-well collection plate, 96-well balance collection plate, Elution plate, 96-well balance elution plate, Elution and balance plate strip caps, Column conditioning buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Digestion Buffer, Enzymatic Stop Solution, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K. DNA purification plate, DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $1,419.00
700005
Chromatrap Mouse Anti-5mC Antibody
Product Code 700005
Product: Chromatrap Mouse Anti-5mC Antibody
Description: 5mC (5-Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease including many cancers.
Category: Monoclonal Antibody
Source: Mouse
Immunogen: Raised against 5-methylcytosine (5mC) in DNA.
Concentration: 2 mg/ml
Form: Purified antibody containing PBS 0.1% sodium azide.
Applications: MeDIP
Purity: Protein G (affinity purified)
Specificity: Human, mouse
Storage: -20°C (Avoid multiple free/thaw cycles)

Our Price: $539.00
700003
Chromatrap Mouse Anti-Histone H3 (tri methyl K9) [Clone 6F12-H4] Antibody
Product Code 700003
Product: Chromatrap Mouse Anti-Histone H3 (tri methyl K9) [Clone 6F12-H4] Antibody
Description: Histone 3 tri methyl K9 (H3K9me3) is a histone mark associated with repressed chromatin. It condenses and compacts the chromatin, restricting the transcription machinery from binding and carrying out gene expression. It is a marker of constitutive heterochromatin, and generally associated with gene silencing.

As a histone mark, it is widely found throughout human cell lines, serving as an abundant antibody target for ChIP. This antibody recognises the synthetic peptide of Human Histone H3, tri methylated at K9.
Category: Monoclonal Antibody
Source: Mouse
Immunogen: Synthetic peptide of Human Histone H3, tri methylated at K9.
Concentration: 1 mg/ml
Form: Purified antibody containing PBS 0.1% sodium azide.
Applications: ChIP, WB, Dot blot, ELISA
Purity: Protein G (affinity purified)
Specificity: Human, Cow. Predicted to detect many other species due to sequence identity such as Mouse, Rat, Rabbit, Hamster, Pig, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster), Non Human Primates
Storage: -20°C (Avoid multiple free/thaw cycles)

Our Price: $469.00
700000
Chromatrap Mouse Anti-Histone H3 Antibody
Product Code 700000
Product: Chromatrap Mouse Anti-Histone H3 Antibody
Description: Histone 3 (H3) one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. It therefore serves as an abundant antibody target for ChIP and recognises the C-terminal peptide of human histone H3.
Category: Monoclonal Antibody
Source: Mouse
Immunogen: Recognizes the C-terminal peptide of human histone H3.
Concentration: 1mg/ml
Applications: ChIP, WB, IP
Purity: Protein G (affinity purified)
Specificity: Mouse, Human, Yeast
Storage: -20°C (Avoid multiple free/thaw cycles)

Our Price: $469.00
700001
Chromatrap Mouse Anti‐RNA polymerase II CTD repeat YSPTSPS (phospho S) Antibody
Product Code 700001
Product: Chromatrap Mouse Anti‐RNA polymerase II CTD repeat YSPTSPS (phospho S) Antibody
Description: RNA polymerase II catalyzes the transcription of DNA into mRNA and other small nuclear RNAs in eukaryotes. It contains a CTD consisting of conserved heptapeptide repeats. Phosphorylation occurs at serine and therine residues located in the CTD repeats to activate the RNA pol II.

RNA pol II binds to several transcription factors in order to initiate transcription and therefore serves as an abundant antibody target for ChIP. This antibody recognises the C-terminal repeat of the largest subunit of RNA polymerase II. It recognises both unphosphorylated and phosphorylated form.
Category: Monoclonal Antibody
Source: Mouse
Immunogen: Recognizes the C-terminal repeat of the largest subunit of RNA polymerase II. It recognises both unphosphorylated and phosphorylated form.
Concentration: 0.8 mg/ml
Form: Purified antibody containing PBS 0.1% sodium azide.
Applications: ChIP, WB, ELISA, ICC, IP, Flow Cytometry
Purity: Protein A (affinity purified)
Specificity: Mouse, Human, Saccharomyces cerevisiae, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Schizososaccharomyces pombe, African Green Monkey
Storage: -20°C (Avoid multiple free/thaw cycles)

Our Price: $469.00
700002
Chromatrap Mouse Anti‐RNA polymerase II CTD repeat YSPTSPS (phospho S5) Antibody
Product Code 700002
Product: Chromatrap Mouse Anti‐RNA polymerase II CTD repeat YSPTSPS (phospho S5) Antibody
Description: RNA polymerase II catalyses the transcription of DNA into mRNA and other small nuclear RNAs in eukaryotes. It contains a CTD consisting of conserved heptapeptide repeats. Phosphorylation occurs at serine and threonine residues located in the CTD repeats to activate the RNA pol II.

RNA pol II binds to several transcription factors in order to initiate transcription and therefore serves as an abundant antibody target for ChIP. This antibody recognises the phosphorylated (Serine 5) form of RNA polymerase II in Human and Saccharomyces cerevisiae.
Category: Monoclonal Antibody
Source: Mouse
Immunogen: Recognizes the phosphorylated (Serine 5) form of RNA polymerase II in Human and Saccharomyces cerevisiae.
Concentration: 0.95 mg/ml
Form: Purified antibody containing PBS 0.1% sodium azide.
Applications: ChIP, WB, ELISA, IF
Purity: Protein A (affinity purified)
Specificity: Human, Saccharomyces cerevisiae
Storage: -20°C (Avoid multiple free/thaw cycles)

Our Price: $469.00
500237
Chromatrap Native ChIP-seq Protein A Kit
Product Code 500237
Product: Chromatrap Native ChIP-seq Protein A Kit
Description: qPCR & Sequencing Chromatrap Native Pro-A ChIP kit for enzymatic shearing only
Background: Chromatrap® Native ChIP kits provide an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction.

In native chromatin the proteins and histones wrapped around DNA are naturally linked and the traditional methods of shearing can easily disrupt the DNA- protein complexes. Therefore the DNA is biologically sheared using enzyme digestion of the chromatin. This kit can be can be used for the study of histone modifications and certain abundant transcription factors that are likely to be bound to DNA.

Chromatrap® Native ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from native chromatin. Using the unique patented technology maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process and has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility, thus more IP assays can be performed from just one sample.
  • The kit is ideal for the enrichment of histone modifications and some low abundant transcription factors.
  • Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
  • The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, N-ChIP Digestion Buffer, ChIP Dialysis Buffer, Resuspension Buffer, Separating Solution 1, Separating Solution 2, Enzymatic Stop Solution, N-ChIP Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer.
Protocol: Download Protocol

Our Price: $739.00
500238
Chromatrap Native ChIP-seq Protein G Kit
Product Code 500238
Product: Chromatrap Native ChIP-seq Protein G Kit
Description: qPCR & Sequencing Chromatrap Native Pro-G ChIP kit for enzymatic shearing only
Background: Chromatrap® Native ChIP kits provide an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction.

In native chromatin the proteins and histones wrapped around DNA are naturally linked and the traditional methods of shearing can easily disrupt the DNA- protein complexes. Therefore the DNA is biologically sheared using enzyme digestion of the chromatin. This kit can be can be used for the study of histone modifications and certain abundant transcription factors that are likely to be bound to DNA.

Chromatrap® Native ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from native chromatin. Using the unique patented technology maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
Features:
  • This kit is compatible for both qPCR and sequencing as a downstream process and has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility, thus more IP assays can be performed from just one sample.
  • The kit is ideal for the enrichment of histone modifications and some low abundant transcription factors.
  • Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
  • The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, N-ChIP Digestion Buffer, ChIP Dialysis Buffer, Resuspension Buffer, Separating Solution 1, Separating Solution 2, Enzymatic Stop Solution, N-ChIP Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA binding buffer, DNA wash buffer and DNA elution buffer.
Protocol: Download Protocol

Our Price: $739.00
500115
Chromatrap Premium ChIP qPCR Protein A Kit
Product Code 500115
Product: Chromatrap Premium ChIP qPCR Protein A Kit
Description: QPCR Premium Chromatrap Pro-A spin column kit for 24 samples (includes control antibodies and primers)
Background: Chromatrap® ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The kits have been optimised for 1000ng sample sizes, which allows for better results from smaller sample sizes and greater flexibility as more IP assays can be performed from one sample.
Features:
  • Designed for qPCR as a downstream process only
  • Suitable for low chromatin loadings (1µg-10µg)
  • Contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitations
  • Up to 10 chromatin preparations by sanitation
  • An enzymatic shearing format is also available
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Hypotonic Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K and 1.5ml Collection tubes. The premium kit also includes Positive Antibody Control: Histone H3, Negative Antibody Control: IgG, Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $519.00
500116
Chromatrap Premium ChIP qPCR Protein G Kit
Product Code 500116
Product: Chromatrap Premium ChIP qPCR Protein G Kit
Description: QPCR Premium Chromatrap Pro-G spin column kit for 24 samples (includes control antibodies and primers)
Background: Chromatrap® ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The kits have been optimised for 1000ng sample sizes, which allows for better results from smaller sample sizes and greater flexibility as more IP assays can be performed from one sample.
Features:
  • Designed for qPCR as a downstream process only
  • Suitable for low chromatin loadings (1µg-10µg)
  • Contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitations
  • Up to 10 chromatin preparations by sanitation
  • An enzymatic shearing format is also available
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Hypotonic Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K and 1.5ml Collection tubes. The premium kit also includes Positive Antibody Control: Histone H3, Negative Antibody Control: IgG, Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $519.00
500167
Chromatrap Premium Enzymatic ChIP qPCR Protein A Kit
Product Code 500167
Product: Chromatrap Premium Enzymatic ChIP qPCR Protein A Kit
Description: QPCR Enzymatic Premium Chromatrap Pro-A spin column kit, 24 coloumns (includes control antibodies and primers)
Background: Chromatrap® Enzymatic ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours.

By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

The Enzymatic ChIP kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40µls) with no DNA clean up steps prior to qPCR. This kit is designed for use as qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1µg-10µg)
Features:
  • Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit contains sufficient Protein-A or Protein-G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and reagents for enzymatic shearing of chromatin (up to 10 preparations)
  • A high throughput format is also available allowing for multiple sample type, antibody and gene targets to be tested in parallel
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Digestion Buffer, Enzymatic Stop Solution, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes. The premium kit also includes Positive Antibody Control: Histone H3, Negative Antibody Control: IgG, Forward and Reverse primer set for GAPDH (human promoter region).
Protocol: Download Protocol

Our Price: $619.00
   
 
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