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Since its foundation in 1982, Dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.

Laboratory professionals in Germany, Austria and Switzerland rely on dianovas expertise for a comprehensive customer support and user friendly solutions in addition to providing high-quality products.

dianova has successfully accompanied biomedical and research manufacturers and successfully launched their products and brands in the German speaking market. As an experienced distribution partner with an excellent reputation in research labs, university research institutions and the pharmaceutical industry, we will support you in launching your products as well as in settling statutory and organizational issues.

In addition to distribution Life Science and Diagnostic Products in the German speaking region Dianova manufactures and markets a series of antibodies with unique properties on a worldwide basis under our own name. To this end dianova has successfully established a distribution network in more than 25 countries.

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DIA-310
Anti-CD31 Monoclonal Antibody
Product Code DIA-310
Product: Anti-CD31 Monoclonal Antibody
Description: Anti-mouse CD31 / DIA-310 Rat monoclonal anti-mouse endothelial cell marker CD31 (PECAM-1), Clone SZ31
Category: Monoclonal Antibody
Species: Rat
Clone: SZ31
Immunogen: Murine amino acid fragment
Isotype: IgG2
Species Reactivity: Mouse, Swine - does not cross-react with rat or human
Specificity: CD31
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections.
CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoetic stem cells and embryonic stem cells. CD31 is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 eliminates these restrictions by allowing high quality immunohistochemical analysis of standard formalin-fixed paraffin sections in mice.
Applications: Immunohistochemistry (standard formalin-fixed paraffin and frozen sections), Western blot
Working Dilutions: 1:20 Immunohistochemistry (IHC)
1:5000 Western Blot
Concentration: 0.2 mg/ml
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: The monoclonal antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of aorta, aortic origin, endocardium, small intestine, colon, brain, lymph nodes, bone marrow, mesenteric vessels, kidney, pancreas, lung, muscle, spinal, liver, adenocarcinoma.
References: 1. Hölscher M, Silter M, Krull S, von Ahlen M, Hesse A, Schwartz P, Wielockx B, Breier G, Katschinski DM, Zieseniss A. Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury. J.Biol.Chem. 286: 11185-11194, 2011
2. Kröger C, Vijayaraj P, Reuter U, Windoffer R, Simmons D, Heukamp L, Leube R, Magin TM. Placental vasculogenesis is regulated by keratin-mediated hyperoxia in murine decidual tissues. Am.J.Pathol. 178(4): 1587-1590, 2011
3. Jiang J, Thyagarajan-Sahu A, Krchnák V, Jedinak A, Sandusky GE, et al. NAHA, a Novel Hydroxamic Acid-Derivative, Inhibits Growth and Angiogenesis of Breast Cancer In Vitro and In Vivo. PLoS ONE 7(3): e34283, 2012
4. Copeland BT, Bowman MJ, Ashman LK. Genetic Ablation of the Tetraspanin CD151 Reduces Spontaneous Metastatic Spread of Prostate Cancer in the TRAMP Model. Mol Cancer Res. 11(1):95-105, 2013
5. Agliano A, Martin-Padura I, Marighetti P, Gregato G, Calleri A, Prior C, Redrado M, Calvo A, Bertolini F. Therapeutic effect of lenalidomide in a novel xenograft mouse model of human blastic NK cell lymphoma/blastic plasmacytoid dendritic cell neoplasm. Clin Cancer Res. 1;17(19):6163-73, 2011
6. Ferretti E, Montagna D, Di Carlo E, Cocco C, Ribatti D, Ognio E, Sorrentino C, Lisini D, Bertaina A, Locatelli F, Pistoia V, Airoldi I. Absence of IL-12Rβ2 in CD33(+)CD38(+) pediatric acute myeloid leukemia cells favours progression in NOD/SCID/IL2RϒC-deficient mice. Leukemia 26(2):225-35,2012

Our Price: $399.00
DIA-303
Anti-CD3 Monoclonal Antibody
Product Code DIA-303
Product: Anti-CD3 Monoclonal Antibody
Description: Anti-mouse CD3 / DIA-303 Rat monoclonal anti-mouse T-cell marker CD3e (TCRE), Clone HH3E
Category: Monoclonal Antibody
Species: Rat
Clone: HH3E
Immunogen: Synthetic peptide from cytoplasmic epitope of CD3: ERPPPVPNPDYEPC
Isotype: Rt IgG1/kappa
Species Reactivity: Mouse, human, canine, feline, equine, bovine, ovine, porcine
Specificity: Murine CD3 epsilon chain (TCRE) (Normal T-cells and T-cell neoplasms)
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone HH3E has been validated specifically for the detection of murine CD3 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). It detects a conserved epitope on the CD3 epsilon chain in a broad variety of species. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. Cluster of differentiation 3 (CD3) is composed of four distinct polypeptide chains CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta, that form a multimeric protein complex. The CD3 complex associates non-covalently with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal trans-duction molecules. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into in-tracellular signals that elicit a change in the cell from a dormant to an activated state. During T cell maturation the expression of CD3 migrates from the cytoplasm of pro-thymocytes to the cell-membrane of thymocytes. The specific appearance at all stages of T cell development make CD3 an ideal marker for normal T cells and T cell neoplasms (lymphomas, leukemias). Moreover, CD3 is a usefull immunohistochemical marker for T cells in tissue sections. In a clinical setting in humans, CD3 serves as an important T cell marker for the classification of malignant lymphomas and leu-kemias. It can also be used to detect T cells in coeliac disease, lymphocytic and collagenous colitis. An anti-CD3 epsilon anti-body (Okt3) has been clinically approved for the induction of immunosuppression in organ transplantation. In animal studies anti-CD3 antibodies can induce tolerance to allografts.
Applications: Immunohistochemistry (standard formalin-fixed paraffin and frozen sections), WB, IP, Flow cytometry
Working Dilutions: IHC 1:100
Concentration: 0.2 mg/ml
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: The monoclonal antibody clone HH3E specifically stains mouse tissue sections by IHC-FFPE: Lymph nodes (A,B), spleen (C) and kidney (D).

All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer and counterstaining with Haematoxylin.
References: 1. Leon F. Flow cytometry of intestinal intraepithelial lymphocytes in celiac disease. Journal of Immunological Methods 363: 177-186, 2011
2. Smith-Garvin JE et al.. T cell activation. Annu Rev Immunol. 27:591-619, 2009
3. Sapp H et al.The terminal ileum is affected in patients with lymphocytic or collagenous colitis. Am J Surg Pathol. 26(11):1484-1492, 2002
4. Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clon-ality by polymerase chain reaction. Vet Immunol Immunopathol. 69:145-164, 1999
5. Mosnier et al. Lymphocytic and collagenous colitis: an immunohistochemical study. Am J Gastroenterol. 91(4):709-713, 1996
6. Chetty R, Gatter K. CD3: Structure, function, and role of immunostaining in clinical practice. The Journal of Pathology 173(4): 303–307, 1994
7. Salvadori S et al. Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J Immunol. 153(11):5176-5182, 1994
8. Nicolls MR et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation 55(3):459-468, 1993

Our Price: $549.00
DIA-404
Anti-CD4 Monoclonal Antibody
Product Code DIA-404
Product: Anti-CD4 Monoclonal Antibody
Description: Anti-mouse CD4 / DIA-404 Rat monoclonal anti-mouse T helper cell marker CD4, Clone GHH4
Category: Monoclonal Antibody
Species: Rat
Clone: GHH4
Immunogen: Recombinant protein (aa 27 - 394 of mouse CD 4)
Isotype: IgG2b kappa
Species Reactivity: Mouse, others not tested.
Specificity: Murine CD4 T helper cells (Th cells)
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone GHH4 has been developed and validated for the detection of murine CD4 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH4 identifies T helper cells in murine FFPE tissue by IHC.
CD4 is a marker for T helper cells. Besides this subpopulation of mature T cells (helper/inducer), CD4 is also expressed on most thymocytes and weakly on macrophages and dendritic cells.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two dif-ferent co-receptors: CD8 and CD4. CD4 is a nonpolymorphous 55 kD glycoprotein belonging to the immunoglobulin superfamily. It acts as a co-receptor with the TCR during antigen-induced T cell activation and thymic differentiation by binding MHC class II and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail.
In humans, CD4 is expressed in the majority of T-cell lymphomas. CD4 plays an important role in the classification of lympho-cytes in inflammatory lesions and malignant lymphomas. CD4 serves as HIV receptor on T cells, macrophages and brain and is downregulated by HIV proteins during AIDS progression.
Applications: Immunohistochemistry (standard formalin-fixed paraffin), others not tested
Working Dilutions: 1:50-1:100 Immunohistochemistry (IHC FFPE)
Concentration: 1 mg/ml
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: Mouse spleen: The monoclonal antibody clone GHH4 specifically stains CD4 in standard FFPE mouse spleen. Incubation with antibody clone GHH4 (10μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with ImmPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Christel Bonnas, Synaptic Systems, Göttingen, Germany).
References: 1. Foti M et al. p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002, 99: 2008-13.
Brady et al. Crystal structure of domains 3 and 4 of rat CD4: relation to the NH2-terminal domains. Science 1993, 260:979-83
2. Janeway CA et al. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992, 10: 645-74.
3. Bierer BE et al. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989, 7: 579-99.
4. Fleischer B et al. Function of the CD4 and CD8 molecules on human T lymphocytes: regulation of T cell triggering. Jour-nal of Immunology 1996, 136: 1625-8
5. Wilde DB et al. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lym-phocyte lines. J Immunol. 1983, 131: 2178-83.
6. Dialynas et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal anti-body GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983, 131: 2445-51.

Our Price: $549.00
DIA-808
Anti-CD8a Monoclonal Antibody
Product Code DIA-808
Product: Anti-CD8a Monoclonal Antibody
Description: Anti-mouse CD8 / DIA-808 Rat monoclonal anti-mouse cytotoxic T cell marker CD8a (CD8 alpha), Clone GHH8
Category: Monoclonal Antibody
Species: Rat
Clone: GHH8
Immunogen: Synthetic peptide from aa 230 - 247 of mouse CD8a (CPRPLVRQEGKPRPSEKIV)
Isotype: IgG2a kappa
Species Reactivity: Mouse, does not cross react with human.
Specificity: Murine CD8a Cytotoxic T-lymphocytes (CTLs)
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone GHH8 has been developed and validated for the detection of murine CD8a in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH8 identifies cytotoxic/suppressor T-cells in murine FFPE tissue by IHC.
CD8 is a marker for T-cells with suppressor and cytotoxic activity. CD8 is also found on the surface of some natural killer cells, most thymocytes, some T-cell lymphomas and large granular lymphocyte leukemias.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two dif-ferent co-receptors: CD8 and CD4. CD8 was first discovered as a cell surface marker in mice and was used to discriminate CD8+ CTLs and CD4+ Th cells. CD8a is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). CD8 alpha beta is expressed on the majority of cytotoxic T-lymphocytes (CTLs). This CD8 heter-omeric proteins (with approx. 32-34 KDa each monomer) are physically associated with a p56 tyrosine kinase to mediate T-cell activation signals through phosphorylation of adjacent proteins.
Besides its important role in T-cell signalling, the CD8 co-receptor is also important for CD8+ T-cell development, stabilisation of the interface between CD8+ T-cells and target cells, and activation of intestinal epithelial T lymphocytes (iIELs). Therefore, CD8 has been the target of many therapeutic strategies designed to modulate T-cell responses during auto-immune reactions, allergy and organ transplant rejection.
Applications: Immunohistochemistry (standard formalin-fixed paraffin), others not tested
Working Dilutions: 1:100 Immunohistochemistry (IHC FFPE)
Concentration: 0.5 mg/ml
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: A, Mouse spleen: The monoclonal antibody clone GHH8 specifically stains CD8a in standard formalin-fixed paraffin-embedded mouse spleen. Incubation with antibody clone GHH8 (5μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with Im-mPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Christel Bonnas, Synaptic Systems, Göttingen, Germany).
B, Mouse liver after viral infection: Specific Detection of invading T-cells with monoclonal antibody clone GHH8 by IHC-FFPE. Note the clear membrane-bound signal free of background (left picture). Detection with a Ventana automated stainer following standard procedures. Incubation for 30min at 4°C with 10μg/ml of antibody clone GHH8, corresponding to a 1:50 dilution (Pic-ture courtesy of Susanne Krasemann, Mouse Pathology Core Facility, UKE, Hamburg, Germany).
References: 1. Kersh AE et al. Cutting Edge: Resident Memory CD8 T Cells Express High-Affinity TCRs. Journal of Immunology 2015, 195: 3520-4.
2. Wu RC et al. New insights on the role of CD8(+)CD57(+) T-cells in cancer. Oncoimmunology 2012, 1: 954-56.
3. Hyland L et al. Mice lacking CD8+ T cells develop greater numbers of IgA-producing cells in response to a respiratory vi-rus infection. Virology 1994, 204: 234-41.
4. Prince HE et al. CD4 and CD8 subsets defined by dual-color cytofluorometry which distinguish symptomatic from asymp-tomatic blood donors seropositive for human immunodeficiency virus. Diagnostic & Clinical Immunology 1987, 5: 188-93.
5. Gaudernack G et al. Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies di-rectly conjugated to monosized magnetic microspheres. Journal of Immunological Methods 1986, 90: 179-87. 6. Fleischer B et al. Function of the CD4 and CD8 molecules on human cytotoxic T lymphocytes: regulation of T cell trigger-ing. Journal of Immunology 1986,136: 1625-8.

Our Price: $549.00
DIA-W09
Anti-IDH1 Monoclonal Antibody
Product Code DIA-W09
Product: Anti-IDH1 Monoclonal Antibody
Description: Anti-pan-IDH1(wt & mutated) / DIA-W09 Rat monoclonal anti-pan-IDH1, Clone W09, to be used as a control for anti-IDH1 R132H
Category: Monoclonal Antibody
Species: Rat
Clone: W09
Immunogen: Peptide sequence 4-120 of human IDH1
Isotype: IgG2a
Species Reactivity: Human
Specificity: Human IDH1, wild type & point mutation
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Isocitrate dehydrogenase (IDH) is an important enzyme in the citric acid cycle and catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate and CO2 while converting NAD+ to NADH. Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. Mouse antibody clone H09 (product number #DIA-H09) reacts specifically with the isocitrate dehydrogenase 1 (IDH1) R132H point mutation in tissue sections from formalin-fixed brain tumor specimens. Therefore this antibody is highly useful for tumor classification. Rat antibody clone W09 detects both wild type (wt) and point mutated IDH1 in glioblastoma (anti-pan-IDH1) and serves as control for the IDH1 point mutation specific mouse antibody clone H09. Moreover, Tan et al. (Mol Cell Proteomics, 2011) have identified IDH1 as a potential diagnostic and prognostic biomarker for Non-small-cell Lung Cancer (NSCLC). These findings suggest that anti-IDH1wt antibody could be used as a histochemical biomarker for prognosis prediction of NSCLC.
Applications: Immunohistochemistry (IHC); Immunohistochemistry (Paraffin-embedded Sections); Western Blot
Working Dilutions: WB 1:500
IHC 1:20
Concentration: 0.2 mg/ml
Control: Antibody control for the IDH1 R132H mutation specific clone H09, (mouse anti-hu IDH1 R132H), cat.no.: DIA-H09
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: A: Strong reaction of IDH1 mutation specific antibody clone H09 in tumor center of anaplastic oligoastrocytoma.
B: Infiltration zone of anaplastic astrocytoma with specific labelling of infiltrating glioma cells by antibody clone H09.
C: Identification of single tumor cells in white matter distant from tumor center with antibody clone H09.
D: Cortex infiltrated by oligodendroglioma with specific labelling of tumor cells by antibody clone H09.
E: Double staining of GFAP (glial fibrillary acidic protein, red) and clone H09 (brown) of oligodendroglioma infiltration zone demonstrating specific labelling of tumor cells but not of GFAP positive reactive astrocytes.
F: Strong reaction of antibody clone H09 with IDH1 R132H mutated diffuse astrocytoma (left) but not with wild type tumor (right).

(pictures courtesy of Prof. Dr. med. Andreas von Deimling, Department of Neuropathology, University Heidelberg/ Clinical Co-operation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany)
References: Capper D, Weissert S, Balss J, Habel A, Meyer J, Jäger D, Ackermann U, Tessmer C, Korshunov A, Zentgraf H, Hartmann C, von Deimling A. Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol. 20(1): 245-254, 2010

Our Price: $599.00
DIA-H09
Anti-IDH1 R132H Monoclonal Antibody
Product Code DIA-H09
Product: Anti-IDH1 R132H Monoclonal Antibody
Description: Anti-IDH1 R132H / DIA-H09 Mouse monoclonal anti-brain tumor marker (Astrocytoma, Oligodendroglioma)
Category: Monoclonal Antibody
Species: Mouse
Clone: H09
Immunogen: Synthetic peptide, amino acid sequence CKPIIIGHHAYGD
Isotype: Mouse IgG2a
Species Reactivity: Human
Form: Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone H09 reacts specifically with the isocitrate dehydrogenase 1 (IDH1) R132H point mutation in tissue sections from formalin-fixed brain tumor specimens. Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. The high frequency and distribution of the IDH1 R132H mutation among specific brain tumor entities allow the highly sensitive and specific discrimination of various tumors by immunohistochemistry, such as anaplastic astrocytoma from primary glioblastoma or diffuse astrocytoma WHO grade II from pilocytic astrocytoma or ependymoma. Noteworthy is the discrimination of the infiltrating edge of tumors with IDH1 mutation from reactive gliosis. This antibody is highly useful for tumor classification and in detecting single infiltrating tumor cells.
Applications: Immunohistochemistry (IHC); Immunohistochemistry (Paraffin-embedded Sections); Western Blot
Working Dilutions: WB 1:500
IHC 1:20
Concentration: 0.2 mg/ml
Positive Control: Oligodendroglioma, diffuse astrocytoma
Negative Control: Pilocytic astrocytoma, primary glioblasto-ma (ca. 95% of cases negative)
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: A: Strong reaction of IDH1 mutation specific antibody clone H09 in tumor center of anaplastic oligoastrocytoma.
B: Infiltration zone of anaplastic astrocytoma with specific labelling of infiltrating glioma cells by antibody clone H09.
C: Identification of single tumor cells in white matter distant from tumor center with antibody clone H09.
D: Cortex infiltrated by oligodendroglioma with specific labelling of tumor cells by antibody clone H09.
E: Double staining of GFAP (glial fibrillary acidic protein, red) and clone H09 (brown) of oligodendroglioma infiltration zone demonstrating specific labelling of tumor cells but not of GFAP positive reactive astrocytes.
F: Strong reaction of antibody clone H09 with IDH1 R132H mutated diffuse astrocytoma (left) but not with wild type tumor (right).

(pictures courtesy of Prof. Dr. med. Andreas von Deimling, Department of Neuropathology, University Heidelberg/ Clinical Co-operation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany)
References: 1. Capper D et al. Monoclonal antibody specific for IDH1 R132H mutation. Acta Neuropathol. 118(5): 599-601, 2009
2. Capper D et al. Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol. 20(1): 245-254, 2010
3. Preusser M et al. IDH testing in diagnostic neuropathology: review and practical guideline article invited by the Euro-CNS research committee. Clinical Neuropathology, 30(5):217-230, 2011
4. Van den Bent MJ et al. Interlaboratory comparison of IDH mutation detection. J Neurooncol 112:173–178, 2013
5. Schumacher T et al. A vaccine targeting mutant IDH1 induces antitumour immunity. Nature 2014, DOI:10.1038/nature13387
6. David NL et al. The 2016 World Health Organization Classification of Tumors of the Central Nervous System: a summary. Acta Neuropathol., 131:803-820, 2016

Our Price: $699.00
DIA-H09-L
Anti-IDH1 R132H Monoclonal Antibody - Ready to Use
Product Code DIA-H09-L
Product: Anti-IDH1 R132H Monoclonal Antibody - Ready to Use
Description: Anti-IDH1 R132H / DIA-H09 Mouse monoclonal anti-brain tumor marker (Astrocytoma, Oligodendroglioma)
Category: Monoclonal Antibody
Species: Mouse
Clone: H09
Immunogen: Synthetic peptide, amino acid sequence CKPIIIGHHAYGD
Isotype: Mouse IgG2a
Species Reactivity: Human
Form: Pre-diluted, Ready to Use, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Reactivity: Antibody clone H09 reacts specifically with the isocitrate dehydrogenase 1 (IDH1) R132H point mutation in tissue sections from formalin-fixed brain tumor specimens. Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. The high frequency and distribution of the IDH1 R132H mutation among specific brain tumor entities allow the highly sensitive and specific discrimination of various tumors by immunohistochemistry, such as anaplastic astrocytoma from primary glioblastoma or diffuse astrocytoma WHO grade II from pilocytic astrocytoma or ependymoma. Noteworthy is the discrimination of the infiltrating edge of tumors with IDH1 mutation from reactive gliosis. This antibody is highly useful for tumor classification and in detecting single infiltrating tumor cells.
Applications: Immunohistochemistry (IHC); Immunohistochemistry (Paraffin-embedded Sections); Western Blot
Positive Control: Oligodendroglioma, diffuse astrocytoma
Negative Control: Pilocytic astrocytoma, primary glioblasto-ma (ca. 95% of cases negative)
Storage: 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images: A: Strong reaction of IDH1 mutation specific antibody clone H09 in tumor center of anaplastic oligoastrocytoma.
B: Infiltration zone of anaplastic astrocytoma with specific labelling of infiltrating glioma cells by antibody clone H09.
C: Identification of single tumor cells in white matter distant from tumor center with antibody clone H09.
D: Cortex infiltrated by oligodendroglioma with specific labelling of tumor cells by antibody clone H09.
E: Double staining of GFAP (glial fibrillary acidic protein, red) and clone H09 (brown) of oligodendroglioma infiltration zone demonstrating specific labelling of tumor cells but not of GFAP positive reactive astrocytes.
F: Strong reaction of antibody clone H09 with IDH1 R132H mutated diffuse astrocytoma (left) but not with wild type tumor (right).

(pictures courtesy of Prof. Dr. med. Andreas von Deimling, Department of Neuropathology, University Heidelberg/ Clinical Co-operation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany)
References: 1. Capper D et al. Monoclonal antibody specific for IDH1 R132H mutation. Acta Neuropathol. 118(5): 599-601, 2009
2. Capper D et al. Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol. 20(1): 245-254, 2010
3. Preusser M et al. IDH testing in diagnostic neuropathology: review and practical guideline article invited by the Euro-CNS research committee. Clinical Neuropathology, 30(5):217-230, 2011
4. Van den Bent MJ et al. Interlaboratory comparison of IDH mutation detection. J Neurooncol 112:173–178, 2013
5. Schumacher T et al. A vaccine targeting mutant IDH1 induces antitumour immunity. Nature 2014, DOI:10.1038/nature13387
6. David NL et al. The 2016 World Health Organization Classification of Tumors of the Central Nervous System: a summary. Acta Neuropathol., 131:803-820, 2016

Our Price: $699.00
   
 
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