General HVD3 ELISA Kit :: 25-Hydroxyvitamin D3 ELISA Kit (CEA915Ge)
General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit
25-Hydroxyvitamin D3 ELISA Kit
|Synonyms:||25-HVD3, 25HVD3, Calcifediol, Calcidiol, H-VD3, 25-Hydroxycholecalciferol, 25-Hydroxy Vitamin D3, 25(OH)D|
|Sensitivity:||The minimum detectable dose of this kit is typically less than 0.92ng/mL|
The standard curve concentrations used for the ELISA's were 200ng/mL, 66.67ng/mL, 22.22ng/mL, 7.41ng/mL, 2.47ng/mL
|Sample Types:||Serum, plasma and other biological fluids|
|Specificity:||This assay has high sensitivity and excellent specificity for detection of 25-Hydroxyvitamin D3 (HVD3). |
No significant cross-reactivity or interference between 25-Hydroxyvitamin D3 (HVD3) and analogues was observed.
|Precision:||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 25-Hydroxyvitamin D3 (HVD3) were tested 20 times on one plate, respectively. |
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 25-Hydroxyvitamin D3 (HVD3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
|Stability:||The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|Procedure Summary:||1. Prepare all reagents, samples and standards;|
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Test Principle:||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B100 (APOB100). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein B100 (APOB100). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein B100 (APOB100), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein B100 (APOB100) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
|Assay Length:||2.5 hours|
|Intended Use:||For Research Use Only|