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2',5'-Oligoadenylate Synthetase 3 (OAS3), Human, ELISA Kit

Cloud-Clone ELISA Kits from ARP American Research Products, Inc.

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Product Code: SEF690HU


Product Details Protocol

Human OAS3 ELISA Kit :: 2',5'-Oligoadenylate Synthetase 3 ELISA Kit (SEF690Hu)

Human 2',5'-Oligoadenylate Synthetase 3 (OAS3) ELISA Kit

Product Code:SEF690Hu

2',5'-Oligoadenylate Synthetase 3 ELISA Kit

Method:Sanwich ELISA
Sensitivity:The minimum detectable dose of this kit is typically less than 0.067ng/mL
Detection Range:0.156-10ng/mL.
The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
Sample Types:Serum, plasma, tissue homogenates and other biological fluids.
Specificity:This assay has high sensitivity and excellent specificity for detection of 2',5'-Oligoadenylate Synthetase 3 (OAS3).
No significant cross-reactivity or interference between 2',5'-Oligoadenylate Synthetase 3 (OAS3) and analogues was observed.
Precision:Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase 3 (OAS3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase 3 (OAS3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability:The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Procedure Summary:1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.
Test Principle:The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 13 (MMP13) level in the sample or standard.
Assay Length:4.5 hours
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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