Rat HPD ELISA Kit :: 4-Hydroxyphenylpyruvate Dioxygenase ELISA Kit (SEE251Ra)
Rat 4-Hydroxyphenylpyruvate Dioxygenase (HPD) ELISA Kit
4-Hydroxyphenylpyruvate Dioxygenase ELISA Kit
|Synonyms:||PPD, 4-HPPD, 4HPPD, GLOD3, Glyoxalase Domain Containing 3, 4-hydroxyphenylpyruvic acid oxidase|
|Sensitivity:||The minimum detectable dose of this kit is typically less than 1.35ng/mL|
The standard curve concentrations used for the ELISA's were 200ng/mL, 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL
|Sample Types:||Tissue homogenates, cell lysates and other biological fluids.|
|Specificity:||This assay has high sensitivity and excellent specificity for detection of 4-Hydroxyphenylpyruvate Dioxygenase (HPD). |
No significant cross-reactivity or interference between 4-Hydroxyphenylpyruvate Dioxygenase (HPD) and analogues was observed.
|Precision:||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested 20 times on one plate, respectively. |
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
|Stability:||The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|Procedure Summary:||1. Prepare all reagents, samples and standards;|
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Test Principle:||This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene C4 (LTC4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene C4 (LTC4) and unlabeled Leukotriene C4 (LTC4) (Standards or samples) with the pre-coated antibody specific to Leukotriene C4 (LTC4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample.|
|Assay Length:||4.5 hours|
|Intended Use:||For Research Use Only|