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Polymerase chain reaction (PCR) is a technique used to amplify specific DNA sequences in vitro. The PCR technique, developed in the early 80’s by Kary Mullis, is a highly efficient method of DNA amplification that has numerous applications and has revolutionised molecular biology. The procedure relies on the use of a thermostable enzyme, DNA polymerase, which can function at temperatures more than 90oC. It also depends on the use of oligonucleotides primers that are designed to bind to the 5’ and 3’ sequences on opposite strands of DNA, flanking specific sites of interest. The basic process of PCR amplification involves a cycle of 1) DNA denaturation, 2) annealing of primers to complementary sequences on the denatured DNA strands and 3) extension of the primers along the DNA fragment, by DNA polymerase, to form two new double strands of DNA. This cycle of denaturation, annealing and extension can be repeated many times resulting in selective enrichment of a DNA sequence in a short time. By amplifying the DNA fragment of interest, PCR enables scientists to study DNA that would otherwise be present in amounts that are undetectable. RNA can also be amplified using PCR.

The PCR technique has been used widely in clinical diagnostics, forensic science and biomedical research. Since the invention of PCR, the technique has been developed further to enable researchers to quantitatively measure PCR product accumulation as it happens in ‘real-time’. The various methods of real-time PCR all rely on the use of fluorescent reporters which can be automatically detected as the DNA is amplified. Real time methods of PCR provide a more accurate and reliable measurement of the amplified PCR product.


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oasig qPCR Mastermix
oasig qPCR Mastermix
Our Price: $219.00

Product Code: oasig-standard-150
Product: oasig qPCR mastermix
Description: The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. They are stable for more than 18 months at ambient temperatures. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.

The performance of the reagents is second to none. We are confident that you will find excellent data quality and even see an improvement in data quality versus many traditional frozen master mixes.

Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.
Product Features: Freeze dried qPCR reagents stored and shipped at ambient temperatures.

A breakthrough product range:
  • No cold chain shipping
  • Simple transport logistics
  • Reduced shipping costs
  • Exceptional data quality
  • Simple protocol
  • Suitable for all real-time PCR machines
  • Intended Use: genesig® products are sold for research use only and are not licensed for diagnostic procedures.
    oasig OneStep qRT-PCR Mastermix
    oasig OneStep qRT-PCR Mastermix
    Our Price: $249.00

    Product Code: oasig-onestep-150
    Product: oasig OneStep qRT-PCR Mastermix
    Description: The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. They are stable for more than 18 months at ambient temperatures. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.

    The performance of the reagents is second to none. We are confident that you will find excellent data quality and even see an improvement in data quality versus many traditional frozen master mixes.

    Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.
    Product Features: Freeze dried qPCR reagents stored and shipped at ambient temperatures.

    A breakthrough product range:
  • No cold chain shipping
  • Simple transport logistics
  • Reduced shipping costs
  • Exceptional data quality
  • Simple protocol
  • Suitable for all real-time PCR machines
  • Intended Use: genesig® products are sold for research use only and are not licensed for diagnostic procedures.
    genesig q16 Extended Warranty - 1 Year
    genesig q16 Extended Warranty - 1 Year
    Our Price: $841.50

    Product Code: genesig-q16-W1
    Product: genesig q16 Extended Warranty - 1 Year
    Description: Our Extended Warranty for the genesig® q16 ensures that you have complete peace of mind for your business. You will be able to continue operating with minimal interuption as we provide a temporary replacement unit while we attended to your instrument.

    The Extended Warranty covers all parts and labour costs and we will arrange for the unit to be transported for any repairs necessary.

    Please note that the Extended Warranty does not include coverage for accidental damage.
    Product Features: Extend the default 1 Year Warranty by another year, for a total of 2 Years Warranty.
    Intended Use: genesig® products are sold for research use only and are not licensed for diagnostic procedures.
    genesig Lab in a box
    genesig Lab in a box
    Our Price: $889.00

    Product Code: genesigEASY-LIAB
    Product: genesig Lab in a box
    Product Features:
  • All that you need to operate the genesig q16
  • Create a DNA testing lab for anyone, anywhere
  • Compact and portable
  • Easy to use
  • Description: Even if you've never performed a DNA test in your life, the genesig q16 makes it affordable and easy to do. If you've never done this kind of testing then you probably don't have a laboratory. That's fine, as our Lab-in-a-box provides all of the simple tools that you'll need to get started

  • A genesig magnetic rack for DNA/RNA extraction
  • Fixed volume, colour-coded pipettes for simple liquid handling
  • Disposable tips for the pipettes
  • Tube racks to hold everything in place whilst you work
  • Digital laboratory timer
  • Intended Use: genesig® products are sold for research use only and are not licensed for diagnostic procedures.
    genesig q16 Extended Warranty - 4 Year
    genesig q16 Extended Warranty - 4 Year
    Our Price: $2,711.50

    Product Code: genesig-q16-W4
    Product: genesig q16 Extended Warranty - 4 Year
    Description: Our Extended Warranty for the genesig® q16 ensures that you have complete peace of mind for your business. You will be able to continue operating with minimal interuption as we provide a temporary replacement unit while we attended to your instrument.

    The Extended Warranty covers all parts and labour costs and we will arrange for the unit to be transported for any repairs necessary.

    Please note that the Extended Warranty does not include coverage for accidental damage.
    Product Features: Extend the default 1 Year Warranty by another 4 years, for a total of 5 Years Warranty.
    Intended Use: genesig® products are sold for research use only and are not licensed for diagnostic procedures.
       
     
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