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Bovine Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA Kit

Bovine Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA Kit


 
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Product Code: KTE10147


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Product CodeKTE10147
Product:Bovine Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA Kit
Description:This Bovine Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA Kit employs a two-site sandwich ELISA to quantitate PYCARD in samples. An antibody specific for PYCARD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYCARD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCARD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCARD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Species:Bovine
Background:PYCARD encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery.The deduced 195-amino acid protein contains an N-terminal pyrin-like domain (PYD) and an 87-residue C-terminal CARD. Fluorescence microscopy demonstrated a ring-like expression in some transfected cells.
Synonyms:PYCARD, ASC, CARD5, MGC10332, TMS, TMS-1, TMS1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:282846
Accession ID:Q8HXK9
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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