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Bovine CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit

Bovine CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit


 
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Product Code: KTE10182


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Product CodeKTE10182
Product:Bovine CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit
Description:This Bovine CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR1A in samples. An antibody specific for PRKAR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Species:Bovine
Background:cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. TSE1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional mutations in this gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.
Synonyms:PRKAR1A, CAR, CNC, CNC1, DKFZp779L0468, MGC17251, PKR1, PPNAD1, PRKAR1, TSE1, cAMP-dependent protein kinase regulatory subunit RIalpha, cAMP-dependent protein kinase type I-alpha regulatory chain, cAMP-depende
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:615074
Accession ID:P00514
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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