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Bovine 28S ribosomal protein S25, mitochondrial (MRPS25) ELISA Kit

Bovine 28S ribosomal protein S25, mitochondrial (MRPS25) ELISA Kit


 
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Product Code: KTE10267


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Product CodeKTE10267
Product:Bovine 28S ribosomal protein S25, mitochondrial (MRPS25) ELISA Kit
Description:This Bovine 28S ribosomal protein S25, mitochondrial (MRPS25) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS25 in samples. An antibody specific for MRPS25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS25 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Species:Bovine
Background:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS25 encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.
Synonyms:MRPS25, DKFZp313H0817, FLJ00023, MRP-S25, RPMS25, mitochondrial 28S ribosomal protein S25
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:533011
Accession ID:P82669
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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