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Bovine Beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB) ELISA Kit

Bovine Beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB) ELISA Kit

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Product Code: KTE10338


Product Details
Product CodeKTE10338
Product:Bovine Beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB) ELISA Kit
Description:This Bovine Beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB) ELISA Kit employs a two-site sandwich ELISA to quantitate LGB in samples. An antibody specific for LGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background:beta-lactoglobulin is the major whey protein of cow's milk and is also present in many other mammalian species. Its structure, properties and biological role have been reviewed many times.Unlike the other main whey protein, alpha-lactalbumin, no clear function has been identified for b-lactoglobulin, although it binds to several hydrophobic molecules, suggesting its role in their transport. The strong suggestion is that the molecule exists primarily as a food source. Several genetic variants have been identified, the main ones in the cow being labelled A and B. Because of its abundance and ease of purification, it has been subjected to a wide range of biophysical studies. Its structure has been determined several times by X-ray crystallography and NMR. beta-lactoglobulin is of direct interest to the food industry since its properties can variously be advantageous or disadvantageous in dairy products and processing .
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:280838
Accession ID:P02754
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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