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Bovine Aspartyl-tRNA synthetase, mitochondrial (DARS2) ELISA Kit

Bovine Aspartyl-tRNA synthetase, mitochondrial (DARS2) ELISA Kit

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Product Code: KTE10371


Product Details
Product CodeKTE10371
Product:Bovine Aspartyl-tRNA synthetase, mitochondrial (DARS2) ELISA Kit
Description:This Bovine Aspartyl-tRNA synthetase, mitochondrial (DARS2) ELISA Kit employs a two-site sandwich ELISA to quantitate DARS2 in samples. An antibody specific for DARS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDARS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DARS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DARS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background:Aspartyl-tRNA synthetase, mitochondrial (DARS2) protein belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). This protein acts as an enzyme. It is known to catalyze the following reaction : ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Synonyms:DARS2, ASPRS, FLJ10514, LBSL, MT-ASPRS, RP3-383J4.2, aspartate tRNA ligase 2, mitochondrial
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Gene ID:538772
Accession ID:A6QPU5
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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