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Bovine Acetyl-CoA Carboxylase Synthetase (ACC) ELISA Kit

Bovine Acetyl-CoA Carboxylase Synthetase (ACC) ELISA Kit


 
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Product Code: KTE10425


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Product Details
 
Product CodeKTE10425
Product:Bovine Acetyl-CoA Carboxylase Synthetase (ACC) ELISA Kit
Description:This Bovine Acetyl-CoA Carboxylase Synthetase (ACC) ELISA Kit employs a two-site sandwich ELISA to quantitate ACC in samples. An antibody specific for ACC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Species:Bovine
Background:Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs ACACAand ACACB.
Synonyms:ACC
Detection Method:Colorimetric
Sample Type:Cell culture supernatants, Serum, Plasma, Other biological fluids
Assay Type:Sandwich ELISA (quantitative)
Intended Use:For Research Use Only
This product is sold for laboratory research use or further manufacturing only and should not be used for human therapeutic or diagnostic applications. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process.

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