14-3-3 zeta, 1-245aa, Human, Recombinant

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Product Name 14-3-3 zeta, 1-245aa, Human, Recombinant
Description The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Background The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Species Human
Synonyms YWHAZ, KCIP-1, MGC111427, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, 14-3-3 zeta, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide 14 3 3 protein zeta/delta, KCIP1, Phospholipase A2, Protein kinase C inhibitor protein 1, Tyr-3/Trp-5 monooxygenase Activation protein, Zeta, Tyr3/Trp5 monooxygenase Activation protein, Zeta
Host E.coli
AA Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN
Tag His-tag
Molecular Weight 28 kDa (246 aa), confirmed by MALDI-TOF.
Form Liquid, in 20mM Tris pH 8.0, 1mM EDTA, 50mM NaCl
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Gannon-Murakami L., et al. (2002) J Biol Chem. 277(26): 23116-23122
Li FQ., et al. (2008) J Cell Biol. 181(7):1141-54
FDA Status For Research Use Only

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