Acid phosphatase 1, 1-158aa, Human, Recombinant

Category: Proteins
Catalog
01-2129
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Product Name Acid phosphatase 1, 1-158aa, Human, Recombinant
Description Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Synonyms Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1.
Host E.coli
Molecular Weight 27.7 kDa (250aa), confirmed by MALDI-TOF.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References De Lorenzo A., et al. (2009) Metabolism.58(3):351-4
Banci M., et al. (2009) Cardiology.113(4):236-242.
Supplier ARP

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