| Product Name | Acidic Fibroblast Growth Factor (aFGF), Human, Recombinant |
|---|---|
| Description | Acidic fibroblast growth factor(aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography. |
| Synonyms | FGF1, FGFA, Acidic Fibroblast Growth Factor (aFGF), HBGF-1, Acidic fibroblast growth factor, aFGF, Beta-endothelial cell growth factor, ECGF-beta, Heparin-binding growth factor 1, Acidic Fibroblast Growth Factor Acidic fibroblast growth factor, AFGF, Beta endothelial cell growth factor, ECGF, ECGF beta, ECGFA, ECGFB, Endothelial Cell Growth Factor alpha, Endothelial Cell Growth Factor beta, FGF 1, FGF alpha, Fibroblast growth factor 1, Fibroblast Growth Factor 1 Acidic, GLIO703, HBGF 1, HBGF1, Heparin binding growth factor 1, Heparin binding growth factor 1 precursor. |
| Host | E.coli |
| Molecular Weight | 14kDa (132aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl pH 7.5, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Jaye M. et al., (1986) Science. 233, 541-544. Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164, 1121-1129. Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 49-54. |
| Supplier | ARP |
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