Anti-CD8a Monoclonal Antibody

Category: Antibodies
Catalog
DIA-808
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Product Name Anti-CD8a Monoclonal Antibody
Description

Antibody clone GHH8 has been developed and validated for the detection of murine CD8a in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH8 identifies cytotoxic/suppressor T-cells in murine FFPE tissue by IHC.
CD8 is a marker for T-cells with suppressor and cytotoxic activity. CD8 is also found on the surface of some natural killer cells, most thymocytes, some T-cell lymphomas and large granular lymphocyte leukemias.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two dif-ferent co-receptors: CD8 and CD4. CD8 was first discovered as a cell surface marker in mice and was used to discriminate CD8+ CTLs and CD4+ Th cells. CD8a is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). CD8 alpha beta is expressed on the majority of cytotoxic T-lymphocytes (CTLs). This CD8 heter-omeric proteins (with approx. 32-34 KDa each monomer) are physically associated with a p56 tyrosine kinase to mediate T-cell activation signals through phosphorylation of adjacent proteins.
Besides its important role in T-cell signalling, the CD8 co-receptor is also important for CD8+ T-cell development, stabilisation of the interface between CD8+ T-cells and target cells, and activation of intestinal epithelial T lymphocytes (iIELs). Therefore, CD8 has been the target of many therapeutic strategies designed to modulate T-cell responses during auto-immune reactions, allergy and organ transplant rejection.

Host Rat
Clone GHH8
Immunogen Synthetic peptide from aa 230 - 247 of mouse CD8a (CPRPLVRQEGKPRPSEKIV)
Isotype IgG2a, kappa
Specificity Murine CD8a Cytotoxic T-lymphocytes (CTLs)
Reactivity Mouse
Applications IHC
Form Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant.
Storage 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Supplier Dianova GmbH

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