Anti-Desmoplakin 1&2 Monoclonal Antibody

Monoclonal Antibodies
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Product Name Anti-Desmoplakin 1&2 Monoclonal Antibody
Description Mouse Monoclonal Antibody to Desmoplakin 1&2. Polypeptides reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000)
Background Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Clone DPI/II 236.23.1
Isotype IgG2b
Immunogen C-terminal polypeptide of Recombinant human desmoplakin (starting at amino acid position 1980)
Synonyms DP, DPI, DPII
Specificity The antibody shows distinct punctate membrane staining of different epithelia. Desmosomes of the myocardium are also positive. Tumors specifically detected: Primary and metastatic carcinoma and meningioma.
Reactivity Bovine, Human, Mouse, Xenopus l
Applications IF, IHC, WB
Form Hybridoma culture supernatant
Positive Control Several human carcinoma cell lines: MCF-7, A-431, HaCaT, CaCo2; bovine cells: MDBK, BMGE
Storage 2-8C
References Cowin, P.et al. (1985). J. Cell Biol. 101: 1442-1454Dockhorn-Dworniczak, B.et al. (1987). Differentiation 35: 53-71Franke, W.W.et al. (1983). Proc. Natl. Acad. Sci., USA, 80: 543-547Moll, R.et al. (1986). Lab. Invest. 54: 4-25Koch, PJ. Et al. (1990) Eur. J. Cell Biol. 53: 1-12Moll, R. et al. (1991) Arch Dermatol Res. 283: 300-309Owaribe, K. et al. (1990) Differentiation 45: 207-220Owaribe, K. et al. (1992) Exp. Cell. Rev 1992: 622-630Schmelz, M. & Franke, WW (1993) Eur. J. Cell Biol. 61: 274-289
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