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|Product Name||BIGH3, 502-683aa, Human, Recombinant|
|Amino Acid Sequence||MGTVMDVLKG DNRFSMLVAA IQSAGLTETL NREGVYTVFA PTNEAFRALP PRERSRLLGD AKELANILKY HIGDEILVSG GIGALVRLKS LQGDKLEVSL KNNVVSVNKE PVAEPDIMAT NGVVHVITNV LQPPANRPQE RGDELADSAL EIFKQASAFS RASQRSVRLA PVYQKLLERM KH|
|Background||BIGH3, also known as TGFBI and Beta ig-h3, is an extracellular matrix protein induced by transforming growth factor(TGF)-beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Recombinant human BIGH3 protein was expressed in E. coli and purified by using conventional chromatography techniques.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Endotoxin Level||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Form||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol|
|Intended Use||For Research Use Only|
|Molecular Weight||36.5 kDa (325aa), confirmed by MALDI-TOF.|
|NCBI Accession #||NP_000349|
|Purity||> 95% by SDS-PAGE|
|References||Billings PC., et al. (2002) J Biol Chem. 277, 28003-9.
Munier FL., et al. (2002) Invest Ophthalmol Vis Sci. 43, 949-54.
Kim JE., et al. (2000) J Biol Chem. 275, 30907-15.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Beta ig-h3, TGFBI, Kerato-epithelin, RGD-CAP, Transforming growth factor-beta-induced protein ig-h3 RGD containing collagen associated protein, Beta ig h3, AI747162, CSD3, Kerato epithelin, LCD1, MGC150270, RGD CAP, TGFBI transforming growth factor, beta induced, 68kDa, AI181842, Beta ig, Big h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, EBMD, Transforming growth factor beta induced protein ig h3.|
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