| Product Name | BIGH3, 502-683aa, Human, Recombinant |
|---|---|
| Description | BIGH3, also known as TGFBI and Beta ig-h3, is an extracellular matrix protein induced by transforming growth factor(TGF)-beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Recombinant human BIGH3 protein was expressed in E. coli and purified by using conventional chromatography techniques. |
| Synonyms | Beta ig-h3, TGFBI, Kerato-epithelin, RGD-CAP, Transforming growth factor-beta-induced protein ig-h3 RGD containing collagen associated protein, Beta ig h3, AI747162, CSD3, Kerato epithelin, LCD1, MGC150270, RGD CAP, TGFBI transforming growth factor, beta induced, 68kDa, AI181842, Beta ig, Big h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, EBMD, Transforming growth factor beta induced protein ig h3. |
| Host | E.coli |
| Molecular Weight | 36.5 kDa (325aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGTVMDVLKG DNRFSMLVAA IQSAGLTETL NREGVYTVFA PTNEAFRALP PRERSRLLGD AKELANILKY HIGDEILVSG GIGALVRLKS LQGDKLEVSL KNNVVSVNKE PVAEPDIMAT NGVVHVITNV LQPPANRPQE RGDELADSAL EIFKQASAFS RASQRSVRLA PVYQKLLERM KH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Billings PC., et al. (2002) J Biol Chem. 277, 28003-9. Munier FL., et al. (2002) Invest Ophthalmol Vis Sci. 43, 949-54. Kim JE., et al. (2000) J Biol Chem. 275, 30907-15. |
| Supplier | ARP |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
