BIGH3, 502-683aa, Human, Recombinant

Proteins, Recombinant
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Product Name BIGH3, 502-683aa, Human, Recombinant
Applications SDS-PAGE
Background BIGH3, also known as TGFBI and Beta ig-h3, is an extracellular matrix protein induced by transforming growth factor(TGF)-beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Recombinant human BIGH3 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Concentration 1 mg/ml (determined by Bradford assay)
Endotoxin Level < 1.0 EU per 1 microgram of protein (determined by LAL method)
Form Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 36.5 kDa (325aa), confirmed by MALDI-TOF.
NCBI Accession # NP_000349
Purity > 95% by SDS-PAGE
References Billings PC., et al. (2002) J Biol Chem. 277, 28003-9.
Munier FL., et al. (2002) Invest Ophthalmol Vis Sci. 43, 949-54.
Kim JE., et al. (2000) J Biol Chem. 275, 30907-15.
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms Beta ig-h3, TGFBI, Kerato-epithelin, RGD-CAP, Transforming growth factor-beta-induced protein ig-h3 RGD containing collagen associated protein, Beta ig h3, AI747162, CSD3, Kerato epithelin, LCD1, MGC150270, RGD CAP, TGFBI transforming growth factor, beta induced, 68kDa, AI181842, Beta ig, Big h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, EBMD, Transforming growth factor beta induced protein ig h3.
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