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October 27, 2017

AAV – a versatile vector for gene therapy

What is gene therapy? Gene therapy is the transfer of genetic material into cells to correct a deficiency in the genome, with the aim of curing […]
June 30, 2017

Matrix effects

Introduction This is the sixth post in a series on ELISA troubleshooting. This one is going to look at ‘matrix effects’. Firstly, what is a matrix […]
March 14, 2017

Elisa troubleshooting tips – High background

Introduction Welcome to our fourth post in a series looking at trouble shooting tips for ELISAs. This post will focus on possible solutions to high background. […]
February 13, 2017

ELISA Troubleshooting tips – High CV

This is the third in a series of blog posts looking at troubleshooting problems with ELISAs. This one is going to focus on the possible causes […]
January 23, 2017

ELISA Troubleshooting tips – No signal

This is the second in a series of blog posts looking at troubleshooting problems with ELISAs. This one is going to focus on the causes and […]
January 9, 2017

ELISA troubleshooting tips – Poor standard curve

This is the first post in a new series looking at ELISA troubleshooting tips – Poor standard curve ELISAs, generally, are pretty straightforward assays to carry […]
November 8, 2016

Sandwich ELISA

Sandwich ELISA This is the third post in a series on ELISA, and this one will focus on the Sandwich ELISA. A sandwich ELISA differs from […]
October 25, 2016

Indirect ELISA Protocol

Indirect ELISA This is the third post in our series of posts and protocols on Enzyme Linked Immunosorbent Assays – ELISAs. The first was a detailed […]
October 6, 2016

How to evenly load protein on western blots

How to evenly load protein on western blots (protein quantitation for western blots) A key point in performing a good and reliable western blot is to […]

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