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What is gene therapy? Gene therapy is the transfer of genetic material into cells to correct a deficiency in the genome, with the aim of curing […]
Introduction This is the sixth post in a series on ELISA troubleshooting. This one is going to look at ‘matrix effects’. Firstly, what is a matrix […]
Introduction Welcome to our fourth post in a series looking at trouble shooting tips for ELISAs. This post will focus on possible solutions to high background. […]
This is the third in a series of blog posts looking at troubleshooting problems with ELISAs. This one is going to focus on the possible causes […]
This is the second in a series of blog posts looking at troubleshooting problems with ELISAs. This one is going to focus on the causes and […]
This is the first post in a new series looking at ELISA troubleshooting tips – Poor standard curve ELISAs, generally, are pretty straightforward assays to carry […]
Sandwich ELISA This is the third post in a series on ELISA, and this one will focus on the Sandwich ELISA. A sandwich ELISA differs from […]
Indirect ELISA This is the third post in our series of posts and protocols on Enzyme Linked Immunosorbent Assays – ELISAs. The first was a detailed […]
How to evenly load protein on western blots (protein quantitation for western blots) A key point in performing a good and reliable western blot is to […]
Direct ELISA This is the second in a series of posts and protocols on Enzyme Linked Immunosorbent Assays (ELISAs). This one deals with the ‘Direct ELISA’. […]
ELISA If you need to measure the concentration or presence of an antigen in solution, then the Enzyme Linked Immunosorbent Assay (ELISA) is one of the workhorses […]
Immunohistochemistry on Frozen tissues IHC Protocol – Frozen Tissue: An introduction This is the second post in a series on immunohistochemistry (IHC). The first post looked at […]
IHC Protocol – paraffin embedded tissues Introduction to IHC-P Put simply, immunohistochemistry (IHC) is a way of staining tissue samples to reveal if and where a specific […]
Synaptopodin Background Synaptopodin is an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot […]
Non-Specific Bands on your Western Blot Sometimes when you look at your blot everything looks pretty much good. A nice strong band where you’re expecting it, […]
Western blot: No signal… Perhaps the biggest and most frustrating issue with a western blot is when you’ve finished everything, and…there’s nothing. No signal at all. […]
What it means when you get multiple bands on your western blot You’ve run the blot. Now it sits in front of you, and looks more […]
Common causes of high background in Western Blotting When your blot comes out looking like a bad x-ray, you’ve got background problems. It’s the result of your […]
Dark spots and white patches on western blot You came in early today, as you really needed the results of this western blot. It’ll determine what […]
A Simple Western Blot Protocol from ARP At its core, a western blot (or ‘immunoblot’) is simply a way of detecting if a specific protein is present […]
Monoclonal Antibodies vs Polyclonal Antibodies: what are the differences and how they will affect your experiments Monoclonal antibodies vs Polyclonal antibodies: The different production processes Both polyclonal […]
