Blog & News

October 3, 2016

Direct ELISA

Direct ELISA This is the second in a series of posts and protocols on Enzyme Linked Immunosorbent Assays (ELISAs). This one deals with the ‘Direct ELISA’. […]
September 21, 2016

General ELISA Protocol

ELISA If you need to measure the concentration or presence of an antigen in solution, then the Enzyme Linked Immunosorbent Assay (ELISA) is one of the workhorses […]
August 30, 2016

IHC Protocol – Frozen Tissue

Immunohistochemistry on Frozen tissues IHC Protocol – Frozen Tissue: An introduction This is the second post in a series on immunohistochemistry (IHC). The first post looked at […]
August 22, 2016

IHC Protocol – Paraffin Embedded Tissue

IHC Protocol – paraffin embedded tissues Introduction to IHC-P Put simply, immunohistochemistry (IHC) is a way of staining tissue samples to reveal if and where a specific […]
August 16, 2016

A Brief Review: Synaptopodin

Synaptopodin Background   Synaptopodin is an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot […]
July 7, 2016

Western Blot troubleshooting: Non-Specific Bands

Non-Specific Bands on your Western Blot Sometimes when you look at your blot everything looks pretty much good. A nice strong band where you’re expecting it, […]
June 21, 2016

Western blot troubleshooting: no signal

Western blot: No signal… Perhaps the biggest and most frustrating issue with a western blot is when you’ve finished everything, and…there’s nothing. No signal at all. […]
June 13, 2016

Western blot troubleshooting: Multiple bands

What it means when you get multiple bands on your western blot You’ve run the blot. Now it sits in front of you, and looks more […]
June 1, 2016

Western blot troubleshooting: high background

Common causes of high background in Western Blotting When your blot comes out looking like a bad x-ray, you’ve got background problems. It’s the result of your […]

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