Cat Growth hormone (GH) ELISA Kit

Catalog
AE62935CA
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Assay Kits, ELISA, Cat ELISA, Abebio
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Product Name Cat Growth hormone (GH) ELISA Kit
Description This assay has high sensitivity and excellent specificity for detection of Cat GH1. No significant cross-reactivity or interference between Cat GH1 and analogues was observed.
Abbreviation GH1
Background Somatotropin is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity.Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus.
Detection Range Request Information
Method Sandwich ELISA
Species Cat
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate GH1 in samples. An antibody specific for GH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Types Serum, Plasma, Other biological fluids.
Sensitivity Request Information
Synonyms GH, GH-N, GHN, IGHD1B, hGH-N, pituitary growth hormone
Uniprot No. P46404

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