| Product Name | EliKine™ Free Triiodothyronine (fT3) ELISA Kit |
|---|---|
| Description | EliKine™ fT3 ELISA Kit has high sensitivity and excellent specificity for detection of fT3. No significant cross-reactivity or interference between fT3 and analogues was observed. Over 99% of Triiodothyronine (T3) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T3 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T3 level changes so that the free T3 concentration remains constant. Thus, measurements of free T3 concentrations correlate more reliably with clinical status than total T3 levels. The increase in total T3 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the free T3 concentration remains basically unchanged. EliKine™ fT3 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT3. A competitive inhibition reaction is launched between fT3 (Standards or samples) and Biotin-conjugated fT3 with the pre-coated antibody specific for fT3. The more amount of fT3 in samples, the less antibody bound by Biotin-conjugated fT3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT3 in the sample. The color development is stopped and the intensity of the color is measured. |
| Detection Range | 2 pmol/L-32 pmol/L |
| Sensitivity | 2.0 pmol/L |
| Reactivity | Bovine, Canine, Chicken, Goat, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep |
| Sample Types | Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Supplier | Abbkine |
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