EliKine™ Mouse IgG ELISA Kit

Category: Assay Kits
Catalog
KET7018
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Product Name EliKine™ Mouse IgG ELISA Kit
Description Immunoglobulin G (IgG) is a multimeric immunoglobulin, built of two heavy chains and two light chains. Each complex has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. EliKine™ Mouse IgG ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IgG in samples. An antibody specific for Mouse IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Mouse IgG present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated anti-mouse IgG antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Mouse IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Range 0.5 ng/mL- 32 ng/mL
Sensitivity 0.5 ng/mL
Reactivity Mouse
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Storage 2-8°C
Supplier Abbkine

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