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|Product Name||CDCA8, 19-280aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAA TADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRL EVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSN RLAQICSSIR THK|
|Background||CDCA8, also known as Borealin, is a component of the chromosomal passenger complex (CPC). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 protein may be required to direct the CPC to centromeric DNA. Deletion of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach. Recombinant human CDCA8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||0.25 mg/ml (determined by BCA assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||18.0 kDa (160aa) confirmed by MALDI-TOF|
|NCBI Accession #||NP_060571|
|Purity||> 90% by SDS-PAGE|
|References||Gassmann R., et al. (2004) J Cell Biol.166:179-191.
Chang J L., et al. (2006) EXP Cell Res. 312:962-973.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Cell division cycle-associated protein 8, Borealin, Dasra-B, PESCRG3|
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