Product Name | CDCA8, 19-280aa, Human |
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Description | CDCA8, also known as Borealin, is a component of the chromosomal passenger complex (CPC). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 protein may be required to direct the CPC to centromeric DNA. Deletion of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach. Recombinant human CDCA8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Synonyms | Cell division cycle-associated protein 8, Borealin, Dasra-B, PESCRG3 |
Host | E.coli |
Molecular Weight | 18.0 kDa (160aa) confirmed by MALDI-TOF |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAA TADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRL EVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSN RLAQICSSIR THK |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl |
Concentration | 0.25 mg/ml (determined by BCA assay) |
Purity | > 90% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Gassmann R., et al. (2004) J Cell Biol.166:179-191. Chang J L., et al. (2006) EXP Cell Res. 312:962-973. |
Supplier | ARP |
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