Chicken Amidophosphoribosyltransferase (PPAT) ELISA Kit

Category: Assay Kits
Catalog
KTE30153
(Ships in 5-10 business days)

Questions? Contact us

Call (800) 832-2611

arp-guarantee
- +
$0.00
More Information
Product Name Chicken Amidophosphoribosyltransferase (PPAT) ELISA Kit
Description Chicken Amidophosphoribosyltransferase (PPAT) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken PPAT. No significant cross-reactivity or interference between Chicken PPAT and analogues was observed. COASY has been shown to interact with P70-S6 Kinase 1. COASY has also been implicated in PI3K signaling, as it was shown to interact with a regulatory subunit of PI3K. Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. This Chicken Amidophosphoribosyltransferase (PPAT) ELISA Kit employs a two-site sandwich ELISA to quantitate PPAT in samples. An antibody specific for PPAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms PPAT, ATASE, GPAT, PRAT, amidophosphoribosyltransferase, glutamine PRPP amidotransferase, glutamine phosphoribosylpyrophosphatate amidotransferase
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Chicken
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 422743
Storage 2-8°C
Supplier Abbkine

All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.