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|Product Name||CHIP, 1-303aa, Human, Recombinant|
|Amino Acid Sequence||MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV EDY|
|Background||CHIP, also known as STUB1, is a cytoplasmic protein whose amino acid sequence is highly conserved across species. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, whereas its U-box domain contains its E3 ubiquitin ligase activity. Its interaction with these molecular chaperones results in client substrate ubiquitylation and degradation by the proteasome. Thus, CHIP acts to tilt the folding-refolding machinery toward the degradative pathway, and it serves as a link between the two. Recombinant human CHIP protein was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Endotoxin Level||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Form||Liquid, in 20mM Tris pH8.0, 1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||27kDa (240aa), confirmed by MALDI-TOF.|
|NCBI Accession #||NP_005852|
|Purity||> 90% by SDS-PAGE|
|References||Kajiro M., et al. (2009) Nat Cell Biol. 11(3):312-9.
Ko HS., et al. (2009) Proc Natl Acad Sci. 106(8):2897-902.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||STUB1, HSPABP2, NY-CO-7, SDCCAG7, UBOX1, Carboxy terminus of HSP70 interacting protein Antigen NY CO 7, CLL associated antigen KW 8, Carboxy terminus of Hsp70p interacting protein, CHIP, E3 ubiquitin protein ligase CHIP, STUB 1, UBOX 1, Heat shock protein A binding protein 2 (c terminal), NY CO 7, Serologically defined colon cancer antigen 7, STIP1 homology and U Box containing protein 1.|
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