| Product Name | CHMP5, 1-219aa, Human |
|---|---|
| Description | CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression. Recombinant human CHMP5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Charged multivesicular body protein 5, C9orf83, CGI-34, HSPC177, PNAS-2, SNF7DC2, Vps60 |
| Host | Hi-5 |
| Molecular Weight | 25.4 kDa (229aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMNRLFG KAKPKAPPPS LTDCIGTVDS RAESIDKKIS RLDAELVKYK DQIKKMREGP AKNMVKQKAL RVLKQKRMYE QQRDNLAQQS FNMEQANYTI QSLKDTKTTV DAMKLGVKEM KKAYKQVKID QIEDLQDQLE DMMEDANEIQ EALSRSYGTP ELDEDDLEAE LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI PAS |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 80% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Howard TL, Stauffer DR, et al. (2001). J Cell Sci. 2395-404. Ward DM, Vaughn MB, et al. (2005). J Biol Chem. 18;280(11):10548-55. Epub 2005 Jan 11. |
| Supplier | ARP |
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